XAS STUDIES OF METAL TRANSFER

金属转移的 XAS 研究

• 批准号: 8170197
• 负责人: Ninian J Blackburn
• 金额: $ 0.34万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170197
• 关键词: Binding; Binding Sites; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Copper; Data; Elements; Funding; Goals; Grant; Institution; Kinetics; Label; Ligand Binding; Location; Measures; Metals; Molecular Chaperones; Monitor; Process; Proteins; Reaction; Research; Research Personnel; Resources; Selenocysteine; Selenomethionine; Source; Spectrum Analysis; United States National Institutes of Health; Work; absorption; cytochrome c oxidase; interest; meetings; member; periplasm

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Detecting and quantifying metal transfer reactions between chaperones and target proteins is currently of great interest. An important experimental requirement is the ability to label one member of the pair in order to track the location of the bound metal as the transfer proceeds. In this proposal we develop a strategy in which selenomethionine (SeM) or selenocysteine (Sec) are substituted for the native Met or Cys ligands of the binding sites of one member of the chaperone target pair. The Se label can then be used as a direct spectroscopic probe of the location of a copper atom during the transfer process via monitoring of the Se-Cu interaction by x-ray absorption spectroscopy at both the Se and Cu absorption edges. Our studies will include copper transfer reactions between (i) the periplasmic efflux proteins CusF and CusB, (ii) T thermophilus cytochrome oxidase CuA and Sco or PCuAC, and (iii) the mammalian CTR1 importer and hCCS. The goal of the work is to obtain evidence for selective transfer chemistry, measure the kinetics of the process, and use this data to interrogate the essential structural elements of the transfer mechanism.

这个子项目是众多研究子项目之一