THE PATHWAY TO HEME-IRON LIBERATION IN STAPHYLOCOCCUS AUREUS

金黄色葡萄球菌释放血红素铁的途径

• 批准号: 8170155
• 负责人: MICHAEL MURPHY
• 金额: $ 0.37万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170155
• 关键词: Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Data; Environment; Funding; Grant; Heme; Heme Iron; Human body; Institution; Iron; Pathway interactions; Research; Research Personnel; Resources; Site-Directed Mutagenesis; Source; Staphylococcus aureus; Surface; System; United States National Institutes of Health; analog; combat; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The human body represents a severely iron-restricted environment for bacterial pathogens. Heme-iron is the most prevalent iron source in the human body and to combat iron-restriction, many bacterial pathogens have developed systems for its utilization. The successful Gram-positive bacterial pathogen, Staphylococcus aureus, employs the recently discovered Iron-regulated surface determinant (Isd) system to acquire heme-iron from its host. Recent structural studies we carried out, using data collected at SSRL, have determined the details of heme binding to key components of the Isd system. The next step is to define the mechanism of heme extraction from host sources, heme transfer, and cytoplasmic heme degradation. The approach is to use x-ray crystallography combined with site directed mutagenesis, heme analog binding and complex formation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 对于细菌病原体来说，人体是一个严格限制铁的环境。血红素铁是人体中最普遍的铁源，为了对抗铁限制，许多细菌病原体已经开发了利用血红素铁的系统。成功的革兰氏阳性细菌病原体金黄色葡萄球菌（Staphylococcus aureus）利用最近发现的铁调节表面决定簇（Isd）系统从其宿主获得血红素铁。我们最近进行的结构研究，使用在SSRL收集的数据，已经确定了血红素结合的ISD系统的关键组成部分的细节。下一步是确定从宿主来源提取血红素，血红素转移和细胞质血红素降解的机制。该方法是使用X射线晶体学结合定点诱变，血红素类似物结合和复合物的形成。