MOLECULAR ARCHITECTURE OF TRANSCRIPTION PREINITIATION COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RNA聚合酶II（PolII）是真核生物中负责合成所有mRNA的酶。在mRNA形成之前，由超过50种蛋白质组成的前起始复合物（preinitiation complex，PIC）围绕PolII成核，以协调启动子解链和转录起始位点选择。了解PIC的分子结构对于理解转录的分子机制以及转录调控至关重要。为了实现这一目标，我们最近组装了一个mini-PIC系统，包括Pol II，TFIIB，TFIIF，TBP和启动子，它们代表了PIC的基本核心组件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA polymerase II (Pol II) is responsible for synthesizing all the mRNA in eukaryote. Before an mRNA is made, a preinitiation complex (PIC) composed of over 50 proteins is nucleated around PolII to orchestrate promoter melting and transcription start site selection. Knowing the molecular architecture of PIC is critical to understand the molecular mechanism of the transcription as well as transcriptional regulation. In order to achieve this goal, we have recently assembled a mini-PIC system consisting of Pol II, TFIIB, TFIIF, TBP and promoter, which represent the essential core components of PIC.