MOLECULAR ARCHITECTURE OF TRANSCRIPTION PREINITIATION COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 RNA聚合酶II(Pol II)负责合成真核生物中所有的mRNA。在合成mRNA之前，在PolII周围形成一个由50多个蛋白质组成的预起始复合体(PIC)，以协调启动子的熔化和转录起始点的选择。了解PIC的分子结构对于理解PIC转录和转录调控的分子机制至关重要。为了实现这一目标，我们最近组装了一个小型PIC系统，由POL II、TFIIB、TFIIF、TBP和启动子组成，它们是PIC的基本核心组件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA polymerase II (Pol II) is responsible for synthesizing all the mRNA in eukaryote. Before an mRNA is made, a preinitiation complex (PIC) composed of over 50 proteins is nucleated around PolII to orchestrate promoter melting and transcription start site selection. Knowing the molecular architecture of PIC is critical to understand the molecular mechanism of the transcription as well as transcriptional regulation. In order to achieve this goal, we have recently assembled a mini-PIC system consisting of Pol II, TFIIB, TFIIF, TBP and promoter, which represent the essential core components of PIC.