STRUCTURAL BIOLOGY OF SELECTED TARGETS FROM MYCOBACTERIUM TUBERCULOSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经从结核分枝杆菌中选择了400多个靶基因进行克隆、表达和晶体结构测定。靶标要么是功能未知的基因产物，要么是参与解毒或氨基酸合成的几个重要途径的酶活性。到目前为止，我们的实验室已经确定了超过25个晶体结构，一些目标已经被克隆、表达和结晶。关于先前确定的晶体结构的结构工作仍在继续，目的是阐明设计有效抑制剂的详细酶机理。该项目需要筛选典型的大量晶体、MAD或SAD分相和高分辨率数据收集，以可视化底物或缓蚀剂结合的细节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have selected more than 400 target genes from Mycobacterium tuberculosis for cloning, expression and crystal structure determination. The targets are either gene products with an unknown function or are enzymatic activities from several essential pathways involved in detoxification or amino acid synthesis. Over 25 crystal structures have been determined in our lab to date and a number of targets have been cloned, expressed and crystallized. Structural work is continuing on the previously determined crystal structures with the goal of elucidating the detailed enzymatic mechanism for the design of effective inhibitors. The project requires the screening of typically large numbers of crystals, MAD or SAD phasing and high-resolution data collection to visualize the details of substrate or inhibitor binding.