STRUCTURAL BIOLOGY OF SELECTED TARGETS FROM M TUBERCULOSIS FOR THE DESIGN OF NO

用于设计 NO 的结核分枝杆菌选定靶标的结构生物学

• 批准号: 7721925
• 负责人: Katherine S Bateman
• 金额: $ 0.17万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721925
• 关键词: Amino Acids; Binding; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Drug Metabolic Detoxication; Funding; Gene Targeting; Goals; Grant; Institution; Mycobacterium tuberculosis; Numbers; Pathway interactions; Phase; Proteins; Research; Research Personnel; Resolution; Resources; Screening procedure; Source; Structure; Tuberculosis; United States National Institutes of Health; Work; design; expression cloning; inhibitor/antagonist; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have selected almost 400 target genes from Mycobacterium tuberculosis for cloning, expression and crystal structure determination. The targets are either gene products with an unknown function or are enzymatic activities from several essential pathways involved in detoxification or amino acid synthesis. 15 crystal structures have been determined to date and a number of targets haven been cloned, expressed and crystallized. Structural work is continuing on the previously determined crystal structures with the goal of elucidating the detailed enzymatic mechanism for the design of effective inhibitors. The project requires the screening of typically large numbers of crystals, MAD or SAD phasing and high-resolution data collection to visualize the details of substrate or inhibitor binding.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们从结核分枝杆菌中筛选出近400个目的基因进行克隆、表达和晶体结构测定。这些靶点要么是功能未知的基因产物，要么是解毒或氨基酸合成中几个重要途径的酶活性。迄今为止，已经确定了15种晶体结构，并且许多靶标已经被克隆、表达和结晶。结构工作正在继续对先前确定的晶体结构，目的是阐明设计有效抑制剂的详细酶促机制。该项目需要筛选通常大量的晶体，MAD或SAD定相和高分辨率数据收集，以可视化底物或抑制剂结合的细节。