GROEL VARIANT: EL43PY

GROEL 变体：EL43PY

• 批准号: 8168594
• 负责人: HAYS S RYE
• 金额: $ 4.3万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168594
• 关键词: Biochemical; Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Research; Research Personnel; Resolution; Resources; Source; Structure; Time; United States National Institutes of Health; Variant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for about a hundred millisec), too short a time for most biochemical and biophysical manipulation. By contrast, our modified GroEL variant appears to undergo this transition at a considerably slower rate. We propose that a high-resolution structure of this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which little is known.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 鉴于野生型凹槽仅短暂地（大约一百毫米），对于大多数生物化学和生物物质操纵而言，野生型凹槽仅在短暂的时间内（约一百毫米），因此，以前的研究无法捕获和研究Groel-Groes复合物的预触发状态并不奇怪。相比之下，我们经过修改的凹槽变体似乎以较慢的速率进行了这种过渡。我们建议，这种新的Groel变体的高分辨率结构利用了NCMI在检查Cryo-Em等分子时所取得的技术进步，很可能会提供前所未有的快速摄像机的groel-Groes机器的关键变构状态，这一点鲜为人知。