GROEL VARIANT: EL43PY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 以前的研究无法捕获和研究GroEL-GroES复合物的预触发状态并不奇怪，因为野生型GroEL仅短暂地填充这种变构状态（约100毫秒），对于大多数生化和生物物理操作来说，时间太短。相比之下，我们的改良GroEL变体似乎以相当慢的速度经历这种转变。我们提出，这种新的GroEL变体的高分辨率结构，利用NCMI在通过cryo-EM检查GroEL等分子方面取得的技术进步，可能会提供GroEL-GroES机器的关键变构状态的前所未有的快照，对此知之甚少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for about a hundred millisec), too short a time for most biochemical and biophysical manipulation. By contrast, our modified GroEL variant appears to undergo this transition at a considerably slower rate. We propose that a high-resolution structure of this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which little is known.