RETROVIRUS RNA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Moloney小鼠白血病病毒(MLV)是一种简单的逆转录病毒，作为病毒组装的模型受到了极大的关注，也是目前在人类基因治疗试验中应用最广泛的载体。像大多数逆转录病毒一样，MLV包装了其基因组的两个副本，正确的包装对病毒的寿命和传染性至关重要。在MLV基因组中已经发现了一个约75个核苷酸的区域，它被认为是一个“核心包裹信号”。该区域形成了一个双发夹基序，其中两组两个茎环结构能够通过环末端的“亲吻作用”形成一个46 kDa的二聚体。我们建议使用CRY-EM来确定“核心封装信号”的结构，这将为基因组二聚化和包装提供精确的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Moloney Murine Leukemia Virus (MLV) is a simple retrovirus that has received considerable attention as a model for virus assembly, and is currently the most widely used vector in human gene therapy trials. Like most retroviruses, MLV packages two copies of its genome, proper packaging of which is critical for viral lifespan and infectivity. A region of about 75 nucleotides, which is believed to act as a "core encapsidation signal," has been identified within the MLV genome. This region forms a double hairpin motif, where two sets of two stem loop structures are capable of forming a 46kDa dimer via "kissing interactions" at the tips of the loops. We propose to use cry-EM to determine the structure of "core encapsidation signal" which will provide the precise mechanisms of genome dimerization and packaging.