RETROVIRUS RNA

逆转录病毒RNA

• 批准号: 8168561
• 负责人: MICHAEL FINLEY SUMMERS
• 金额: $ 1.08万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168561
• 关键词: Attention; Computer Retrieval of Information on Scientific Projects Database; Crying; Dimerization; Funding; Genome; Grant; Human; Institution; Longevity; Modeling; Moloney Leukemia Virus; Murine leukemia virus; Nucleotides; Research; Research Personnel; Resources; Retroviridae; Signal Transduction; Source; Structure; United States National Institutes of Health; Viral; Virus Assembly; dimer; gene therapy; stem; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Moloney Murine Leukemia Virus (MLV) is a simple retrovirus that has received considerable attention as a model for virus assembly, and is currently the most widely used vector in human gene therapy trials. Like most retroviruses, MLV packages two copies of its genome, proper packaging of which is critical for viral lifespan and infectivity. A region of about 75 nucleotides, which is believed to act as a "core encapsidation signal," has been identified within the MLV genome. This region forms a double hairpin motif, where two sets of two stem loop structures are capable of forming a 46kDa dimer via "kissing interactions" at the tips of the loops. We propose to use cry-EM to determine the structure of "core encapsidation signal" which will provide the precise mechanisms of genome dimerization and packaging.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 莫洛尼鼠白血病病毒（MLV）是一种简单的逆转录病毒，作为病毒组装的模型受到了相当大的关注，目前是人类基因治疗试验中使用最广泛的载体。像大多数逆转录病毒一样，MLV包装其基因组的两个拷贝，适当的包装对病毒寿命和感染性至关重要。在MLV基因组中已经鉴定出一个约75个核苷酸的区域，据信该区域充当“核心腺苷酸化信号”。该区域形成双发夹基序，其中两组两个茎环结构能够在环的尖端通过“接吻相互作用”形成46 kDa二聚体。 我们建议使用cry-EM来确定“核心折叠信号”的结构，这将提供基因组二聚化和包装的精确机制。