RETROVIRUS RNA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 莫洛尼鼠白血病病毒（MLV）是一种简单的逆转录病毒， 作为病毒组装的模型，受到了相当大的关注，并且是目前使用最广泛的 人类基因治疗试验中的载体。像大多数逆转录病毒一样，MLV将其 基因组，其适当包装对于病毒寿命和感染性至关重要。的区域 大约75个核苷酸，被认为是作为“核心腺苷酸化信号”， 在MLV基因组中鉴定。该区域形成双发夹基序，其中两组 的两个茎环结构能够通过“接吻相互作用”形成46 kDa的二聚体， 环的尖端。 我们建议使用cry-EM来确定的结构？核心 发射信号？这将提供基因组二聚化的精确机制， 包装.

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Moloney Murine Leukemia Virus (MLV) is a simple retrovirus that has received considerable attention as a model for virus assembly, and is currently the most widely used vector in human gene therapy trials. Like most retroviruses, MLV packages two copies of its genome, proper packaging of which is critical for viral lifespan and infectivity. A region of about 75 nucleotides, which is believed to act as a 'core encapsidation signal,' has been identified within the MLV genome. This region forms a double hairpin motif, where two sets of two stem loop structures are capable of forming a 46kDa dimer via 'kissing interactions' at the tips of the loops. We propose to use cry-EM to determine the structure of ?core encapsidation signal? which will provide the precise mechanisms of genome dimerization and packaging.