RETROVIRUS RNA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 莫洛尼鼠白血病病毒(MLV)是一种简单的逆转录病毒，它已经收到了 作为一种备受关注的病毒组装模型，也是目前应用最广泛的 人类基因治疗试验中的载体。像大多数逆转录病毒一样，MLV包装了两个拷贝的ITS 基因组的正确包装对病毒的寿命和传染性至关重要。一个地区 大约75个核苷酸被认为起到了“核心包裹信号”的作用 在MLV基因组中鉴定。这一区域形成了一个双重发夹图案，其中两套 的茎环结构能够通过亲吻相互作用形成46 kDa的二聚体。 圆圈的尖端。我们建议使用CRY-EM来确定堆芯的结构 封装信号？它将提供基因组二聚化的精确机制和 包装。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Moloney Murine Leukemia Virus (MLV) is a simple retrovirus that has received considerable attention as a model for virus assembly, and is currently the most widely used vector in human gene therapy trials. Like most retroviruses, MLV packages two copies of its genome, proper packaging of which is critical for viral lifespan and infectivity. A region of about 75 nucleotides, which is believed to act as a 'core encapsidation signal,' has been identified within the MLV genome. This region forms a double hairpin motif, where two sets of two stem loop structures are capable of forming a 46kDa dimer via 'kissing interactions' at the tips of the loops. We propose to use cry-EM to determine the structure of ?core encapsidation signal? which will provide the precise mechanisms of genome dimerization and packaging.