KV CHANNEL IN LIPID MEMBRANES (2D CRYSTALS OF KVAP)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的目的是获得脂质膜中电压门控钾通道的结构。Kv通道至少有两种不同的构象，我们希望在5-9埃分辨率下获得这两种结构，以便我们可以推断二级结构。如果条件允许，我们将在更高的分辨率下追求结构，这将揭示已被证明对电压门控至关重要的强脂质-蛋白质相互作用的结构细节。当前的目标是获得目前可用的2D晶体形式的3D结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project will be aimed at obtaining structures of a voltage-gated potassium (Kv) channel in lipid membranes. There are at least two distinct conformations for a Kv channel and we want to obtain both structures at 5-9 angstrom resolutions so that we can deduce the secondary structures. If conditions permit, we will pursue structures at higher resolutions that will reveal the structural details for the strong lipid-protein interaction that has been demonstrated to be critical for voltage-gating. The immediate goal is to obtain the 3D structure of a 2D crystal form that is currently available.