An 8K camera for the cryoEM at UT Southwestern Medical Center

UT 西南医学中心用于冷冻电镜的 8K 摄像机

• 批准号: 8052603
• 负责人: Qiu-Xing Jiang
• 金额: $ 59.97万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052603
• 关键词: Area; Biological; Biomedical Research; Cellular biology; Controlled Environment; Cryoelectron Microscopy; Data Collection; Electron Microscope; Electrons; Equipment; Film; Funding; Goals; Housing; Image; Imaging Techniques; Institution; Maintenance; Medical center; Microscope; Molecular Biology; Research; Resources; Sampling; Services; Specimen; Techniques; Time; United States National Institutes of Health; cost; instrument

DESCRIPTION (provided by applicant): We propose to purchase a grade B 8k x 8k Tietz F816 camera, which will serve as a main data- collection medium in the high-end electron cryo-microscope (cryoEM; a JEOL2200FS FEG) at UT Southwestern Medical Center. The cryoEM at UT Southwestern is the only high-end electron microscope in the Dallas / Ft. Worth area that is dedicated to the imaging of biological specimens. The requested camera will replace the low-end cameras currently installed in the microscope, will eliminate the use of film almost completely, and will enable more efficient data- collection from a variety of biological samples. It will greatly reduce the cost of using film and other resources, and will remove most film-related contamination in the microscope. With the currently available cameras, the one scope on our campus is not able to support all projects described in this proposal. The proposed camera will enable the efficient use of one scope for multiple projects because it will allow users to make better use of the scope time and largely eliminate film-related down-time. Ten research groups on the UT Southwestern campus will utilize the requested camera. They form a strong user group with projects that cover a broad spectrum of biomedical research. These projects will fully utilize the requested equipment for data collection in our cryoEM. In addition, UT Southwestern has a strong commitment in establishing cryoEM as a major imaging technique for molecular and cell biology. It has supported the purchase, maintenance, and services of the current cryoEM. It is now constructing a new facility with better-controlled environments for housing one or two more high-end cryoEM instruments, which will extend the application of cryoEM techniques to many other NIH-funded projects on our campus. With the added capability of supporting multiple projects in one cryoEM, the requested camera will not only be beneficial to multiple NIH-funded projects, but also allow UT Southwestern to reach its goal of becoming a premier institution in applying cryoEM to biomedical research. PUBLIC HEALTH RELEVANCE: The proposed purchase of an 8k x 8k camera will greatly improve the data-collection efficiency in the electron cryo-microscope at UT Southwestern Medical Center. It will enable the cryo-microscope to well support multiple NIH-funded projects that are oriented at important health-related questions.

描述（由申请人提供）：我们建议购买一台B级8 k x 8 k Tietz F816相机，该相机将作为UT西南医学中心高端冷冻电子显微镜（cryoEM; JEOL 2200 FS FEG）的主要数据收集介质。UT西南的cryoEM是达拉斯/英尺唯一的高端电子显微镜。值得一提的是，专门用于生物标本成像的区域。所要求的相机将取代目前安装在显微镜中的低端相机，将几乎完全消除胶片的使用，并将能够从各种生物样品中更有效地收集数据。它将大大降低使用胶片和其他资源的成本，并将消除显微镜中大部分与胶片有关的污染。根据目前可用的摄像机，我们园区的一个范围无法支持本提案中描述的所有项目。拟议的摄像机将使一个范围有效地用于多个项目，因为它将使用户能够更好地利用范围的时间，并在很大程度上消除与电影有关的停机时间。UT西南校区的十个研究小组将使用所要求的相机。他们形成了一个强大的用户群，其项目涵盖了广泛的生物医学研究。这些项目将充分利用所要求的设备在我们的cryoEM中进行数据收集。此外，UT Southwestern致力于将cryoEM建立为分子和细胞生物学的主要成像技术。它支持当前cryoEM的购买、维护和服务。它现在正在建设一个新的设施，具有更好的控制环境，用于容纳一个或两个高端cryoEM仪器，这将把cryoEM技术的应用扩展到我们校园内许多其他NIH资助的项目。随着在一个cryoEM中支持多个项目的能力的增加，所要求的相机不仅有利于多个NIH资助的项目，而且还允许UT Southwestern实现其成为将cryoEM应用于生物医学研究的首要机构的目标。 公共卫生关系：拟议购买的8 k x 8 k相机将大大提高UT西南医学中心电子冷冻显微镜的数据收集效率。它将使冷冻显微镜能够很好地支持多个NIH资助的项目，这些项目面向重要的健康相关问题。