KV CHANNEL IN LIPID MEMBRANES (2D CRYSTALS OF KVAP)

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的目的是获得脂质膜中电压门控钾通道的结构。Kv通道至少有两种不同的构象，我们希望在5-9埃分辨率下获得这两种结构，以便我们可以推断二级结构。如果条件允许，我们将在更高的分辨率下追求结构，这将揭示已被证明对电压门控至关重要的强脂质-蛋白质相互作用的结构细节。当前的目标是获得目前可用的2D晶体形式的3D结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project will be aimed at obtaining structures of a voltage-gated potassium (Kv) channel in lipid membranes. There are at least two distinct conformations for a Kv channel and we want to obtain both structures at 5-9 Angstrom resolution so that we can deduce the secondary structures. If conditions permit, we will pursue structures at higher resolutions that will reveal the structural details for the strong lipid-protein interaction that has been demonstrated to be critical for voltage-gating. The immediate goal is to obtain the 3D structure of a 2D crystal form that is currently available.