INTERACTIONS BETWEEN PHOSPHO-ACCEPTING AND KINASE DOMAIN OF ECOLI CHEA

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 激酶结构域P4将磷酸基团转移到组氨酸激酶CheA的P1结构域中的保守组氨酸残基。在这项工作中，我们的目标是确定这两个域之间的相互作用。以前的实验已经确定，P1域是广泛的相互分离，并有相当大的自由度。为了解决相关的长距离，我们使用来自大肠杆菌的氘代蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The kinase domain P4, transfers a phosphate group to the conserved histidine residue in the P1 domain of histidine kinase CheA. In this work we aim to determine the interaction between these two domains. Previous experiments have established that P1 domains are widely separated from each other and have considerable degree of freedom. In order to resolve the associated long distances, we use deuterated proteins from E.coli.