CRYSTALLOGRAPHIC STUDIES OF LARGE MACROMOLECULAR ASSEMBLIES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质和核酸及其形成的复合体的结构研究是我们正在进行的研究的主题，这些复合体涉及执行克里克的分子生物学中心法则。目前正在研究的结构包括细菌复制体组分与DNA的复合体和Tau片段的复合体；大肠杆菌RNA聚合酶与DNA的复合体；没有配体的CAP和通过RNA聚合酶与DNA结合的CAP；新型氨基酰tRNA合成酶与底物的复合体；70年代的核糖体与底物、因子或抗生素的复合体的结构；50S核糖体亚基与抗生素的复合体；真核核糖体的结构研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural studies of proteins and nucleic acids and the complexes they make that are involved in executing Crick's Central Dogma of Molecular Biology are the subjects of our ongoing studies. The structures being pursued include complexes between the components of the bacterial replisome - DnaB helicase complexed with primase and DNA as well as complexes with a fragment of Tau; E. coli RNA polymerase complexed with DNA; CAP without ligands and CAP with cAMP bound to DNA with RNA polymerase; novel aminacyl-tRNA synthetases complexed with substrates; structures of the 70S ribosome complexed with substrates, factors, or antibiotics; complexes between the 50S ribosomal subunit and antibiotics; structural studies of the eukaryotic ribosomes.