RNA POLYMERASE

RNA聚合酶

• 批准号: 8363339
• 负责人: THOMAS Arthur STEITZ
• 金额: $ 0.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363339
• 关键词: Binding; Cells; Complex; Crystallography; DNA; DNA-Directed RNA Polymerase; Escherichia coli; Funding; Goals; Grant; Holoenzymes; Individual; Light; National Center for Research Resources; Principal Investigator; RNA; RNA chemical synthesis; RNA polymerase sigma 70; Research; Research Infrastructure; Resources; Sigma Factor; Source; Synchrotrons; Transcription Initiation; United States National Institutes of Health; Work; cost; melting; promoter; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. RNA polymerase is one of the largest protein complexes in the cell and is responsible for transcribing RNA using DNA as templates. Although the core complex can bind non-specific DNA, it requires an appropriate sigma factor to form the holoenzyme for specific promoter binding and transcription initiation. Upon binding to a promoter, the sigma factor facilitates melting of the downstream DNA to form the transcription initiation bubble, and thus converting the RNA polymerase to an open complex competent for initiating RNA synthesis. The goal of this project is to capture the individual steps of transcription initiation. The holoenzyme we are working on is the E. coli RNA polymerase with bound primary sigma factor (sigma-70).

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 RNA聚合酶是细胞中最大的蛋白质复合物之一，负责使用DNA作为模板转录RNA。虽然核心复合物可以结合非特异性DNA，但它需要适当的σ因子来形成全酶以用于特异性启动子结合和转录起始。在与启动子结合后，σ因子促进下游DNA的解链以形成转录起始泡，并因此将RNA聚合酶转化为能够起始RNA合成的开放复合物。这个项目的目标是捕捉转录起始的各个步骤。我们正在研究的全酶是E。大肠杆菌RNA聚合酶与结合的初级σ因子（σ-70）。