FREEZING 70S CRYSTALS FROM THERMUS THERMOPHILUS UNDER PRESSURE

在压力下冷冻来自嗜热菌的 70S 晶体

• 批准号: 8363566
• 负责人: THOMAS Arthur STEITZ
• 金额: $ 1.14万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363566
• 关键词: Binding; Clinical; Data; Freezing; Funding; Grant; Liquid substance; National Center for Research Resources; Nitrogen; Pharmaceutical Preparations; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Ribosomes; Source; Thermus thermophilus; United States National Institutes of Health; cost; pressure; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In order to localize small clinical relevant drugs bound to the 70S ribosome, we are striving to collect the highest resolution of diffraction data possible. During our experiments we noticed that our crystals are very sensitive to the way they are frozen. We are hoping that freezing our crystal in liquid nitrogen under pressure will allow us to gain higher quality of diffraction data.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 为了定位与70 S核糖体结合的临床相关的小药物，我们正在努力收集尽可能高分辨率的衍射数据。在我们的实验中，我们注意到我们的晶体对它们的冷冻方式非常敏感。 我们希望在压力下将我们的晶体冷冻在液氮中将使我们能够获得更高质量的衍射数据。