CRYSTALLOGRAPHIC STUDIES OF RNA-PROTEIN MACHINES

RNA-蛋白质机器的晶体学研究

• 批准号: 8361690
• 负责人: THOMAS Arthur STEITZ
• 金额: $ 4.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361690
• 关键词: Binding; Complex; Eukaryota; Funding; Grant; National Center for Research Resources; Oryctolagus cuniculus; Peptide Elongation Factor 2; Principal Investigator; Process; Protein Biosynthesis; Proteins; RNA; Research; Research Infrastructure; Resolution; Resources; Reticulocytes; Ribosomes; Role; Signal Recognition Particle; Site; Son of Sevenless Proteins; Source; Structure; Transfer RNA; Translations; United States National Institutes of Health; X-Ray Crystallography; Yeasts; base; cost; polypeptide; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The projects being pursued try to establish the structural bases for the functional mechanisms by which the ribosome and associated factors achieve their roles in protein synthesis by high resolution X-ray crystallography. To illuminate the mechanisms of each step in the process of protein synthesis, we wish to obtain crystals of the ribosome trapped in as many of the steps in the protein synthesis cycle as possible and to establish their structures at the highest resolution possible. The complexes whose structures will be pursued include those of the 1) 70S ribosome with bound m-RNA, tRNA and elongation factors, 2) complexes between the 70S ribosomal and a P-site tRNA containing a translation arresting polypeptide, and 3) complexes between the 70S ribosome the signal recognition particle. To explore the differences between the bacterial ribosome and its much large counterpart from eukaryotes, particularly in the initiation step of protein synthesis, the structures of the 40S subunit, the 60S subunit and/or the SOS ribosome, including an initiation complex formed with IRIS RNA, and using ribosomes isolated from either yeast or rabbit reticulocyte will be pursued.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 所追求的项目试图建立核糖体和相关因素通过高分辨率X射线晶体学在蛋白质合成中的作用的功能机制的结构碱基。为了阐明蛋白质合成过程中每个步骤的机制，我们希望获得尽可能多的蛋白质合成周期中捕获的核糖体晶体，并以最高分辨率的最高分辨率建立其结构。将追求结构的复合物包括1）70S核糖体具有结合的M-RNA，tRNA和伸长因子的核心体，2）70S核糖体和一个含有含有翻译的多肽翻译的p-site tRNA之间的络合物，以及70s核糖体之间的复合物。探索细菌核糖体与其来自真核生物的大量对应物之间的差异，尤其是在蛋白质合成的启动下，40S亚基的结构，60S亚基和/或SOS核糖体，包括与Iris RNA形成的启动复合物，并使用核糖体从Yeast或Rabbit retbit Will will will will will will will will will will will will will will will will will will will will will will。