THE IDENTIFICATION OF SMC-5 ASSOCIATED PROTEINS IN C ELEGANS BY CO-IMMUNOPRECIP

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 指导人体内每个细胞功能的信息被编码在 基因组DNA。基因组DNA的适当组织有助于损伤的修复 从环境侮辱中获得的DNA，并确保其准确分布到 体内的每一个细胞。组织基因组DNA的基本需要在许多人中是相同的 更简单的生物或有机体。许多需要保存的细胞因子 在这些更简单的有机体中也发现了人类基因组的完整性，包括一组 称为染色体(或SMC)蛋白质结构维持的因素。这个 拟议的研究检查SMC-5和SMC-6蛋白在基因组中的功能 稳定性。我们正在研究土生蛔虫的这一重要生物学过程， 秀丽隐杆线虫，非常适合细胞和发育研究 流程。我们对线虫的初步研究表明了这些SMC的重要性 促进基因组稳定性的蛋白质。最有趣的是，我们找到了证据证明 对这些SMC蛋白在细胞中产生卵母细胞和 精子。鉴于SMC-5和SMC-6蛋白在人类中的保守性，信息 从这些研究中获得的成果将提高我们对类比人类如何 蛋白质可能会影响人类的生育能力和出生缺陷。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The information directing the function of each cell within the human body is encoded in genomic DNA. The proper organization of genomic DNA facilitates the repair of damage to the DNA sustained from environmental insults and to ensure its accurate distribution to every cell within the body. The essential need to organize genomic DNA is shared in many simpler living creatures or organisms. Many of the cellular factors needed to preserve human genomic integrity are also found in these simpler organisms, including a set of factors termed the Structural Maintenance of Chromosomes (or SMC) proteins. The proposed research examines the function for the SMC-5 and SMC-6 proteins in genomic stability. We are examining this vital biological process in the soil-dwelling roundworm, Caenorhabditis elegans, which is ideally suited for studies of cellular and developmental processes. Our preliminary studies in C. elegans have shown the importance for these SMC proteins in promoting genomic stability. Most interestingly, we found evidence for an important requirement for these SMC proteins in the cells that give rise to oocytes and sperms. Given the conservation of SMC-5 and SMC-6 proteins in human, the information gained from these studies will improve our understanding of how the analogous human proteins may impact human fertility and birth defects.