THE IDENTIFICATION OF SMC-5 ASSOCIATED PROTEINS IN C ELEGANS BY CO-IMMUNOPRECIP

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 指导人体内每个细胞功能的信息被编码在 基因组DNA 基因组DNA的适当组织有助于修复损伤， DNA持续从环境的侮辱，并确保其准确分布， 体内的每一个细胞。 组织基因组DNA的基本需要在许多生物中都有， 更简单的生物或有机体。 许多需要保存的细胞因子 人类基因组完整性也存在于这些简单的生物体中，包括一组 称为染色体结构维持（或SMC）蛋白的因子。 的 拟议的研究检查了SMC-5和SMC-6蛋白在基因组中的功能， 稳定 我们正在研究土壤中的蛔虫的这一重要生物过程， 秀丽隐杆线虫，这是理想的细胞和发育的研究， 流程. 我们对C.线虫已经证明了这些SMC的重要性， 蛋白质促进基因组稳定性。 最有趣的是，我们发现了 这些SMC蛋白在产生卵母细胞的细胞中的重要要求， 精子 鉴于SMC-5和SMC-6蛋白在人体中的保守性， 从这些研究中获得的信息将提高我们对类似人类如何 蛋白质可能会影响人类的生育能力和出生缺陷。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The information directing the function of each cell within the human body is encoded in genomic DNA. The proper organization of genomic DNA facilitates the repair of damage to the DNA sustained from environmental insults and to ensure its accurate distribution to every cell within the body. The essential need to organize genomic DNA is shared in many simpler living creatures or organisms. Many of the cellular factors needed to preserve human genomic integrity are also found in these simpler organisms, including a set of factors termed the Structural Maintenance of Chromosomes (or SMC) proteins. The proposed research examines the function for the SMC-5 and SMC-6 proteins in genomic stability. We are examining this vital biological process in the soil-dwelling roundworm, Caenorhabditis elegans, which is ideally suited for studies of cellular and developmental processes. Our preliminary studies in C. elegans have shown the importance for these SMC proteins in promoting genomic stability. Most interestingly, we found evidence for an important requirement for these SMC proteins in the cells that give rise to oocytes and sperms. Given the conservation of SMC-5 and SMC-6 proteins in human, the information gained from these studies will improve our understanding of how the analogous human proteins may impact human fertility and birth defects.