THE IDENTIFICATION OF SMC-5 ASSOCIATED PROTEINS IN C ELEGANS BY CO-IMMUNOPRECIP

联合免疫法鉴定线虫中 SMC-5 相关蛋白

• 批准号: 7957779
• 负责人: Raymond C Chan
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957779
• 关键词: Adult; Affect; Biological Process; Biology; Caenorhabditis elegans; Cells; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; DNA; Defect; Development; Developmental Process; Ensure; Fertility; Funding; Fungal Genome; Genome Stability; Genomics; Grant; Human; Human body; Infertility; Institution; Life; Maintenance; Nematoda; Oocytes; Organism; Proteins; Research; Research Personnel; Resources; Soil; Source; United States National Institutes of Health; improved; offspring; repaired; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The information directing the function of each cell within the human body is encoded in genomic DNA. The proper organization of genomic DNA facilitates the repair of damage to the DNA sustained from environmental insults and to ensure its accurate distribution to every cell within the body. The essential need to organize genomic DNA is shared in many simpler living creatures or organisms. Many of the cellular factors needed to preserve human genomic integrity are also found in these simpler organisms, including a set of factors termed the Structural Maintenance of Chromosomes (or SMC) proteins. The proposed research examines the function for the SMC-5 and SMC-6 proteins in genomic stability. We are examining this vital biological process in the soil-dwelling roundworm, Caenorhabditis elegans, which is ideally suited for studies of cellular and developmental processes. Our preliminary studies in C. elegans have shown the importance for these SMC proteins in promoting genomic stability. Most interestingly, we found evidence for an important requirement for these SMC proteins in the cells that give rise to oocytes and sperms. Defects in SMC-5 and SMC-6 caused infertility in adult worms and increased the likelihood for developmental defects in the offspring from the affected adult worms. Given the conservation of SMC-5 and SMC-6 proteins in human, the information gained from these studies will improve our understanding of how the analogous human proteins may impact human fertility and birth defects.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 指导人体内每个细胞功能的信息编码在基因组DNA中。 基因组DNA的正确组织有助于修复环境损伤对DNA的损伤，并确保其准确分布到体内的每个细胞。 组织基因组DNA的基本需要在许多简单的生物或有机体中共享。 许多保持人类基因组完整性所需的细胞因子也存在于这些简单的生物体中，包括一组称为染色体结构维持（或SMC）蛋白的因子。 拟议的研究检查了SMC-5和SMC-6蛋白在基因组稳定性中的功能。 我们正在研究这一重要的生物学过程中的土居蛔虫，秀丽隐杆线虫，这是非常适合研究细胞和发育过程。 我们对C. elegans已经显示了这些SMC蛋白在促进基因组稳定性中的重要性。 最有趣的是，我们发现了这些SMC蛋白在产生卵母细胞和精子的细胞中的重要需求的证据。 SMC-5和SMC-6的缺陷导致蠕虫不育，并增加了受影响蠕虫后代发育缺陷的可能性。 鉴于SMC-5和SMC-6蛋白在人类中的保守性，从这些研究中获得的信息将提高我们对类似人类蛋白质如何影响人类生育力和出生缺陷的理解。