IDENTIFICATION OF THE DROSOPHILA F-BOX PROTEIN PALLBEARER SUBSTRATE(S)

果蝇 F-BOX 蛋白 PALLBEARER 底物的鉴定

• 批准号: 8171444
• 负责人: Nathalie Claudine Franc
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171444
• 关键词: Age related macular degeneration; Apoptotic; Autoimmune Diseases; Blindness; Boxing; Cells; Co-Immunoprecipitations; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Drosophila genus; F-Box Proteins; Failure; Funding; Goals; Grant; Immune response; Immunity; Institution; Laboratories; Lupus; Mass Spectrum Analysis; Nerve Degeneration; Organism; Play; Proteins; Research; Research Personnel; Resolution; Resources; Role; Source; United States National Institutes of Health; novel; ubiquitin-protein ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptotic cell clearance is crucial to the development and the resolution of immune responses in all multicellular organisms. Failure to clear apoptotic cells can trigger autoimmune diseases such as Lupus, and neurodegeneration, such as age-related macular degeneration, the most frequent cause of blindness. We have identified a novel Drosophila F-Box protein that plays a role in apoptotic cell clearance (Silva et al, 2007. Immunity 27 (4): 585-96). This F-Box protein acts together with SkpA, Lin19 and Effete to form a SCF complex with E3 ligase activity. The goal of this project is to couple co-ImmunoPrecipitation and Mass Spectrometry to identify potential Pallbearer substrate(s), which will then be further characterized in our laboratory.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 凋亡细胞清除对于所有多细胞生物体中免疫应答的发展和解决至关重要。未能清除凋亡细胞可能引发自身免疫性疾病，如狼疮和神经变性，如年龄相关性黄斑变性，这是最常见的致盲原因。 我们已经鉴定了一种在凋亡细胞清除中起作用的新型果蝇F-Box蛋白（Silva等人，2007. Immunity 27（4）：585-96）。该F-Box蛋白与SkpA、Lin 19和Effete一起作用以形成具有E3连接酶活性的SCF复合物。该项目的目标是将共免疫沉淀和质谱法结合起来，以识别潜在的Pallbearer底物，然后在我们的实验室中对其进行进一步表征。