STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 应激激素脱落酸对拟南芥在干旱胁迫下的生存至关重要。关键的脱落酸信号转导蛋白具有YFP融合，将用于纯化蛋白质复合体，用于分析信号转导伙伴的质谱学。这项研究将导致识别新的进一步候选蛋白，这些蛋白在早期脱落酸信号转导中发挥作用。鉴定出的蛋白质将进一步与利用Affymetrix Genechips完成的保卫细胞特定基因组规模的基因表达分析进行比较。基因敲除实验将验证质谱学鉴定的蛋白质的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The stress hormone abscisic acid is crucial to survival of Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and will be used to purify protein complexes for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new further candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.