STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 胁迫激素脱落酸对拟南芥在干旱胁迫下的存活至关重要。关键的脱落酸信号转导蛋白具有YFP融合体，并且具有纯化的复合物，用于使用质谱法分析信号转导伴侣。这项研究将导致识别新的候选蛋白，在早期脱落酸信号转导功能。将进一步将鉴定的蛋白质与已经使用Affyssin Genechips完成的基因表达的保卫细胞特异性基因组规模分析进行比较。基因敲除实验将验证质谱鉴定的蛋白质的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The stress hormone abscisic acid is crucial to survival in Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and have had complexes purified for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.