STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

拟南芥应激激素信号转导

• 批准号: 8365831
• 负责人: JULIAN I SCHROEDER
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365831
• 关键词: Abscisic Acid; Arabidopsis; Biology; Cells; Complex; Droughts; Funding; Fungal Genome; Gene Expression; Genomics; Grant; Hormones; Lead; Mass Spectrum Analysis; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Signal Transduction; Source; Stress; United States National Institutes of Health; cost; knockout gene; protein function; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The stress hormone abscisic acid is crucial to survival in Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and have had complexes purified for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 应激激素脱落酸对拟南芥在干旱胁迫下的生存至关重要。关键的脱落酸信号转导蛋白具有YFP融合，并具有纯化的复合体，用于使用质谱学分析信号转导伙伴。这项研究将导致识别新的候选蛋白，这些蛋白在早期脱落酸信号转导中发挥作用。鉴定出的蛋白质将进一步与利用Affymetrix Genechips完成的保卫细胞特定基因组规模的基因表达分析进行比较。基因敲除实验将验证质谱学鉴定的蛋白质的功能。