CONSTRUCTION OF A FLEXIBLE HYPERPOLARIZATION SYSTEM

柔性超极化系统的构建

• 批准号: 8171655
• 负责人: MATTHEW E MERRITT
• 金额: $ 5.23万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171655
• 关键词: 2,4-Dinitrophenol; Cell Nucleus; Chemicals; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Detection; Eye; Frequencies; Funding; Grant; Institution; Journals; Magnetic Resonance; Mammals; Mind; Nuclear; Physics; Physiologic pulse; Protons; Published Comment; Pyruvic Acid; Research; Research Personnel; Resources; Sampling; Source; System; United States National Institutes of Health; Universities; Washington; base; cryostat; design; flexibility; in vivo; instrument; magnetic field; research study; solid state nuclear magnetic resonance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A flexible dynamic nuclear polarization instrument is under construction at the RR. It is planned to be a more flexible instrument than the commercially-available Oxford HyperSense DNP system. Further, the terms and conditions of purchase of the HyperSense prohibit in vivo experiments. With an eye towards potential large mammal in vivo applications, the system is being designed around a larger sample container that will hold approximately twice the amount of sample as the HyperSense sample cup. The DNP system will be based on a cryostat designed in collaboration with Mark Conradi of Washington University placed in a Bruker 15cm bore magnet energized to a field of ~4.6 Tesla. Recent results have demonstrated that incremental increases in field strength can yield dramatically higher nuclear polarizations.(1,2) With this in mind, we have designed the new system to operate at an ESR frequency of ~129 GHz. Furthermore, the extra space available in the large bore magnet should allow the incorporation of a double resonance probe for 1H and either 13C or 89Y. Cross-polarization solid state NMR pulse sequences can then be used to transfer polarization from high gamma protons, which polarize much more quickly under DNP than lower gamma species, to the detection nucleus.(3) References for construction of a polarizer: 1. Jannin S, Comment A, Kurdzesau F, Konter JA, Hautle P, van den Brandt B, van der Klink JJ. A 140 GHz prepolarizer for dissolution dynamic nuclear polarization. The Journal of Chemical Physics 2008;128(24):241102-241104. 2. J¿hannesson H, Macholl S, Ardenkjaer-Larsen JH. Dynamic Nuclear Polarization of [1-13C]pyruvic acid at 4.6 tesla. Journal of Magnetic Resonance 2009;197(2):167-175. 3. Maly T, Debelouchina GT, Bajaj VS, Kan-Nian H, Chan-Gyu J, Mak-Jurkauskas ML, Sirigiri JR, van der Wel PCA, Herzfeld J, Temkin RJ, Griffin RG. Dynamic nuclear polarization at high magnetic fields. Journal of Chemical Physics 2008;128(5):N.PAG.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RR正在建造一个灵活的动态核极化仪。 它计划成为比市售Oxford HyperSense DNP系统更灵活的仪器。此外，购买HyperSense的条款和条件禁止进行体内实验。 着眼于潜在的大型哺乳动物体内应用，该系统围绕更大的样品容器进行设计，该容器将容纳大约两倍于HyperSense样品杯的样品量。DNP系统将基于与华盛顿大学的Mark Conradi合作设计的低温恒温器，该低温恒温器放置在布鲁克15 cm内径的磁体中，通电至~4.6特斯拉的磁场。最近的结果表明，场强的增量增加可以产生显着更高的核极化。（1，2）考虑到这一点，我们设计了在约129 GHz的ESR频率下工作的新系统。此外，大口径磁体中可用的额外空间应允许结合用于1H和13 C或89 Y的双共振探针。然后，交叉极化固态NMR脉冲序列可以用于将极化从高伽马质子转移到检测核，高伽马质子在DNP下比低伽马物质更快地极化。(3)构造偏振器的参考文献：1。Jannin S，Comment A，Kurdzesau F，Konter JA，Hautle P，货车den Brandt B，货车der Klink JJ.溶解动态核极化的140 GHz预极化器。化学物理学报2008;128（24）：241102-241104. 2. J <$hannesson H，Macholl S，Ardenkjaer-Larsen JH. [1- 13 C]异烟酸在4.6特斯拉下的动态核极化。磁共振杂志2009;197（2）：167-175。3. Maly T，Debelouchina GT，Bajaj VS，Kan-Nian H，Chan-Gyu J，Mak-Jurkauskas ML，Boggiri JR，货车der Wel PCA，Herzfeld J，Temkin RJ，Griffin RG.强磁场下的动态核极化。化学物理学报2008;128（5）：N. PAG.