EXTRACTING WIRING DIAGRAMS FROM NEURONAL CIRCUITS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 神经元回路的接线图可以从通过神经组织连续切割的切片（<50 nm）的电子显微镜（EM）图像获得。在哺乳动物的大脑中，即使是局部电路也可能跨越数百到数千微米的空间。以纳米分辨率跨越数百微米的图像消耗许多千兆字节的存储。我们正在与PSC的人员合作开发一个高吞吐量管道，用于处理和分析这些非常大的数据集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The wiring diagrams of neuronal circuits may be obtained from electron microscopy (EM) images of serially cut, ultrathin (<50 nm) sections through nervous tissue. In the mammalian brain, even local circuits may span hundreds to thousands of microns in space. Images spanning hundreds of microns at nanometer resolution consume many gigabytes of storage. We are working with people at PSC to develop a high throughput pipeline for processing and analysis of these very large data sets.