Functional and cell-type specific axonal pathways in the primate brain

灵长类动物大脑中的功能和细胞类型特异性轴突通路

基本信息

  • 批准号:
    10272370
  • 负责人:
  • 金额:
    $ 153.57万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-09-01 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract Over the past decade, there have been transformative advances in three areas of mammalian neuroscience. First, our ability to record from large populations of neurons has dramatically increased with the advent of new electrode technologies and improved multiphoton imaging. Second, the study of brain connections in their entirety, connectomics, has come into its own as a field. Most recently, there has been a revolution in the classification of neuronal types, largely by probing which genes are translated into RNA in cells (transcriptomics). What is lacking is a way to bring these three fields together, particularly in the studies of non-human primates and humans. The goal of the current RFA is to create innovative tools for use in humans and non-human primates. In particular, two suggested topics are to develop: (1) “novel methods for tagging individual neurons such that cellular components of a functional circuit can be explored” and (2) “innovative approaches to bridge scales of experimental approach. Studies that can explore molecular and cellular mechanisms of neural activity in broader contexts are encouraged.” To achieve these goals, we present an innovative approach to characterize neuronal cell types in macaques and humans, combining transcriptomics, inter-areal connectivity and functional studies at multiple scales, from individual neurons to entire brains. We will build the necessary tools to create integrated atlases of individual brains that combine six modalities into a common reference frame: (1) functional MRI, to measure functional properties of brain areas at 0.5-1 mm resolution, (2) widefield optical imaging, to map bulk neuronal activity at the cortical surface with ~100 µm resolution, (3) multiphoton calcium imaging, to map neuronal activity in individual neurons across multiple cortical areas, (4) diffusion tensor imaging (DTI), to map axonal tracts in the white matter with 0.5-1 mm resolution, (5) “axonal connectomics”, to map projections of individual myelinated axons from efferent cell bodies to their postsynaptic targets, and (6) multiplexed FISH, to assay transcriptomic identities of the same cells whose physiology and projection targets have been defined. Data from first three modalities will be collected, starting with in vivo studies of macaques and correlated with subsequent analysis of brain tissue with the last three modalities. Only the last three steps will be used in the study of human brain tissue, although functional MRI could, in principle, be obtained from research institutions with appropriate programs for prospective studies.
项目总结/文摘

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

R CLAY REID其他文献

R CLAY REID的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('R CLAY REID', 18)}}的其他基金

Functional and cell-type specific axonal pathways in the primate brain
灵长类动物大脑中的功能和细胞类型特异性轴突通路
  • 批准号:
    10653987
  • 财政年份:
    2021
  • 资助金额:
    $ 153.57万
  • 项目类别:
Viral Strategies for Functional Connectomics in the Visual System
视觉系统中功能性连接组学的病毒策略
  • 批准号:
    10231175
  • 财政年份:
    2017
  • 资助金额:
    $ 153.57万
  • 项目类别:
Viral Strategies for Functional Connectomics in the Visual System
视觉系统中功能性连接组学的病毒策略
  • 批准号:
    9751980
  • 财政年份:
    2017
  • 资助金额:
    $ 153.57万
  • 项目类别:
Large-scale connectivity and function in a cortical circuit
皮质回路中的大规模连接和功能
  • 批准号:
    8300070
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
Large-scale connectivity and function in a cortical circuit
皮质回路中的大规模连接和功能
  • 批准号:
    8656161
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
Large-scale connectivity and function in a cortical circuit
皮质回路中的大规模连接和功能
  • 批准号:
    8179783
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
EXTRACTING WIRING DIAGRAMS FROM NEURONAL CIRCUITS
从神经元电路中提取接线图
  • 批准号:
    8364225
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
Large-scale connectivity and function in a cortical circuit
皮质回路中的大规模连接和功能
  • 批准号:
    8452709
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
Large-scale connectivity and function in a cortical circuit
皮质回路中的大规模连接和功能
  • 批准号:
    8779970
  • 财政年份:
    2011
  • 资助金额:
    $ 153.57万
  • 项目类别:
EXTRACTING WIRING DIAGRAMS FROM NEURONAL CIRCUITS
从神经元电路中提取接线图
  • 批准号:
    8171802
  • 财政年份:
    2010
  • 资助金额:
    $ 153.57万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了