FUNCTIONAL MICRO-ORGANIZATION OF COLOR AND ORIENTATION IN PRIMARY VISUAL CORTEX

初级视觉皮层颜色和方向的功能性微观组织

• 批准号: 8172864
• 负责人: R CLAY REID
• 金额: $ 6.58万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172864
• 关键词: Calcium; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Electrophysiology (science); Funding; Goals; Grant; Image; Institution; Knowledge; Laser Scanning Microscopy; Literature; Maps; Neurons; Ocular Physiology; Primates; Property; Research; Research Personnel; Resolution; Resources; Sampling; Signal Transduction; Source; Techniques; Time; United States National Institutes of Health; Work; area striata; cytochrome c oxidase; novel strategies; optical imaging; response; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The principal goal of this study is to understand the functional organization of neurons in layers 2/3 of primary visual cortex (V1). We are examining the organization of cells with defined response properties, both in their relation to larger compartments like cytochrome-oxidase blobs and orientation maps, as well as in their fine-scale arrangement. These issues have been studied in the past with two approaches: electrophysiological recordings, which yield functional characterizations of single neurons, and intrinsic signal imaging, which provides an overview of functional maps in cortex but with very low resolution (less than 100 m). The key aspect of our present work is that we are using a new approach, calcium imaging with two-photon laser-scanning microscopy, which bridges the gap between single-unit electrophysiology and conventional optical imaging. The technique gives us single cell resolution from an essentially complete sample of cells in a given patch of cortex, and, to our knowledge, this is the first time it has been used to study cortical microarchitecture in primates. Our initial results have revealed an intriguing and very clear clustering of color-selective cells into functional units, most likely related to cytochrome-oxidase blobs. Within these color clusters we find an even more detailed organization, with blue ON cells segregated from blue OFF cells, and with groups of blue/yellow cells arranged in clusters distinct from red/green cells. These results could not have been predicted from earlier work, and they provide a framework for resolving some long-standing debates in the visual physiology literature.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的主要目的是了解初级视皮层（V1）2/3层神经元的功能组织。 我们正在研究具有确定的响应特性的细胞的组织，无论是在它们与细胞色素氧化酶斑点和方向图等更大的隔间的关系中，还是在它们的精细尺度排列中。 这些问题在过去已经研究了两种方法：电生理记录，产生单个神经元的功能特性，和内在信号成像，提供了一个概述的功能地图在皮层，但分辨率很低（小于100米）。 我们目前工作的关键方面是，我们正在使用一种新的方法，钙成像与双光子激光扫描显微镜，桥梁之间的差距差距单单位电生理学和传统的光学成像。 该技术为我们提供了从给定皮层中基本完整的细胞样本中获得单细胞分辨率的方法，据我们所知，这是第一次将其用于研究灵长类动物的皮层微结构。 我们的初步结果揭示了一个有趣的和非常清晰的聚类颜色选择性细胞的功能单位，最有可能与细胞色素氧化酶斑点。 在这些颜色簇中，我们发现了更详细的组织，蓝色ON细胞与蓝色OFF细胞分离，并且蓝色/黄色细胞组排列在不同于红色/绿色细胞的簇中。 这些结果不能从早期的工作中预测，他们提供了一个框架，解决视觉生理学文献中一些长期存在的争论。