IMAGING LUNG CANCER IN MICE TO IMPROVE RADIATION THERAPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 探讨microCT作为肺癌定量标志物的实用性。作为正在进行的K 08资助的一部分，我们将研究多种疗法在这种原发性肺癌模型中的疗效，目标如下： 具体目标1-本项目将帮助建立扩展研究所需的后勤支持，以支持本K 08。更具体地说，该项目将作为一种工具，为未来的研究制定动物转移和IACUC。这也将提供一种简化门控方案的工具。目前，动物被插管用于扫描同步通气并用ECG监测。我们建议使用脉搏血氧仪作为一种微创方法，将简化收购。 特定目标2-先前的工作@ MGH已经收集了合理的图像数据，没有心脏和呼吸门控。我们的假设是，缺乏这种支持会导致高估肿瘤体积。通过比较有和没有门控的采集，我们将具体确定门控对肿瘤体积的影响。这是一个生存协议，将作为一个工具，以强调我们的生存支持技能。 具体目标3 -组织学证据表明，这些肿瘤的血管密度约为体积的15%。我们假设双扫描（有/无血池剂）将使我们能够定量血管密度和密度变化。本项目将测试这一假设，并为R 01应用程序奠定基础。 具体目标4 -到目前为止，microPET已被认为是检测小肿瘤的金标准。我们假设，对于小鼠，microCT的分辨率可能足够高，以提供超出当前金标准的更高水平的灵敏度（和特异性）。这也将为计划的R 01应用程序提供支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Explore the utility of microCT as a quantiative marker for lung cancer. As part of an ongoing K08 grant we will be studying the efficacy of a number of therapies in this model of primary lung cancer, with these goals: Specific Aim 1- this project will help establish the logistical support required for the extended studies in support of this K08. More specifically the project will serve as a vehicle to work out animal transfer and IACUC for future studies. This will also provide a vehicle to streamline the gating protocols. Currently animals are intubated for scan synchronous ventilation and monitored with ECG. We propose the use of a pulse oximeter as a less invasive method that will streamline the acquisition. Specific Aim 2- previous work @ MGH has gathered reasonable image data without cardiac and respiratory gating. Our hypothesis is that the absence of this support leads to overestimation of tumor volumes. By comparing the acquisition with and without gating, we will determine specifically the impact of gating on tumor volume. This is a survival protocol and will serve as a vehicle to stress our survival support skills. Specific Aim 3 - Histologic evidence suggests that these tumors will have vascular densities of ~15% of volume. We hypothesize that a dual scan (with/without blood pool agent) will allow us to quantitate vascular density and changes in that density. This project will test that hypothesis and lay the groundwork for an R01 application. Specific Aim 4 - to this point , microPET has been considered a gold standard for detection of small tumors. We hypothesis that for the mouse, the resolution of the microCT might be sufficiently high to provide an increased level of sensitivity (and specificity) beyond that of the current gold standard. This too will provide support for a planned R01 application.