MULTI-SPECTRAL IMAGING OF ATHEROSCLEROSIS

动脉粥样硬化的多光谱成像

• 批准号: 8171611
• 负责人: KETAN B Ghaghada
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171611
• 关键词: Animals; Apolipoprotein E; Arterial Fatty Streak; Atherosclerosis; Calcium Signaling; Cells; Computer Retrieval of Information on Scientific Projects Database; Contrast Media; Development; Diagnosis; Disease; Funding; Goals; Grant; Human; Image; Inflammation; Inflammatory; Institution; Iodine; Life; Liposomes; Mus; Patients; Protocols documentation; Research; Research Personnel; Resources; Rupture; Scanning; Screening procedure; Signal Transduction; Site; Source; Specimen; Staging; Time; United States National Institutes of Health; calcification; macrophage; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Scans will be performed at energies ranging between 80 - 140 kVp. These are ApoE -/- mice specimens---the standard mouse model used in the study atherosclerosis. The development of atherosclerosis in humans involve formation of calcifications and accumulation of inflammatory cells, such as macrophages, at sites of plaque. A major challenge in the diagnosis of vulnerable from non-vulnerable plaque is to identify the extent of inflammation and calcification. The goal of this project is to investigate the feasibility of multi-spectral imaging for differentiating iodine-loaded macrophages from calcifications in atherosclerotic plaques. Preliminary studies will be performed in specimens of mice models of atherosclerosis (ApoE mice). The specific aims are: 1. Imaging of control mice specimens at different energy settings. We will try to answer the following questions: a) how does calcification signal (HU) vary as a function of kVp; b) how does iodine signal vary as a function of kVp; c) can we differentiate iodine signal from calcification signal ? 2. Imaging of iodine-loaded ApoE mice: ApoE mice will be injected with liposomal-iodine. The animals will be sacrificed at later time points (all of the live animal studies will be performed in Houston under approval animal protocol). The mice specimens will then undergo multi-spectral micro-CT imaging. We will try to answer the following questions: a) Can we separate iodine signal from calcium signal within the plaque; b) Can we quantify iodine signal and correlate it with the number of macrophages present in the plaque ? The ability to differentiate iodine signal from calcium signal could have facilitate development of contrast agents for differentiating stable from'rupture-prone' atherosclerotic plaques. This would have huge implications in the screening of patients who are at late stage of the disease.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 扫描能量范围为 80 - 140 kVp。这些是 ApoE -/- 小鼠标本——动脉粥样硬化研究中使用的标准小鼠模型。 人类动脉粥样硬化的发展涉及钙化的形成和炎症细胞（例如巨噬细胞）在斑块部位的积累。诊断易损斑块与非易损斑块的主要挑战是确定炎症和钙化的程度。该项目的目标是研究多光谱成像区分载碘巨噬细胞与动脉粥样硬化斑块钙化的可行性。初步研究将在动脉粥样硬化小鼠模型（ApoE 小鼠）样本中进行。具体目标是： 1. 对照小鼠标本在不同能量设置下的成像。 我们将尝试回答以下问题：a) 钙化信号 (HU) 作为 kVp 的函数如何变化； b) 碘信号如何随 kVp 变化； c) 我们可以区分碘信号和钙化信号吗？ 2.载碘ApoE小鼠的成像：将脂质体碘注射到ApoE小鼠中。这些动物将在稍后的时间点被处死（所有活体动物研究将根据批准的动物协议在休斯顿进行）。然后对小鼠样本进行多光谱显微 CT 成像。 我们将尝试回答以下问题：a）我们能否将斑块内的碘信号与钙信号分开？ b) 我们可以量化碘信号并将其与斑块中存在的巨噬细胞数量相关联吗？ 区分碘信号和钙信号的能力可以促进用于区分稳定和“易破裂”动脉粥样硬化斑块的造影剂的开发。这将对疾病晚期患者的筛查产生巨大影响。