DEVELOPMENT OF CONFOCAL RAMAN MICROSCOPE FOR LIVE CELL IMAGING

用于活细胞成像的共焦拉曼显微镜的开发

• 批准号: 8170414
• 负责人: JEON WOONG KANG
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170414
• 关键词: Cells; Cellular Structures; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Grant; Image; Institution; Lasers; Life; Malaria; Maps; Microscope; Microscopy; Refractive Indices; Research; Research Personnel; Resolution; Resources; Signal Transduction; Source; System; Techniques; United States National Institutes of Health; Variant; base; cancer cell; cellular imaging

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Confocal Raman microscopy is a powerful technique for chemical imaging mainly because of its ability to provide chemical composition with high spatial resolution. Raman mapping of a cell were done by researchers. However, imaging live cell is still challenging because of the weak Raman signal strength. Confocal Raman microscopy will be developed based on NIR laser. This system can be used to study malaria-infected RBC and cancer cells as well as to find a relation between sub cellular structure and other biophysical parameters such as refractive index variations inside of a cell.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 共焦拉曼显微镜是一种强大的化学成像技术，主要是因为它能够提供具有高空间分辨率的化学成分。细胞的拉曼图谱是由研究人员完成的。然而，由于拉曼信号强度较弱，活细胞成像仍然具有挑战性。共焦拉曼显微技术将在近红外激光的基础上发展。该系统可用于研究疟疾感染的红细胞和癌细胞，以及发现亚细胞结构和其他生物物理参数之间的关系，如细胞内的折射率变化。