DEVELOPMENT OF CONFOCAL RAMAN MICROSCOPE FOR LIVE CELL IMAGING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 共聚焦拉曼显微镜是一种强大的化学成像技术，主要是因为它能够提供高空间分辨率的化学成分。研究人员对细胞进行了拉曼成像。然而，由于拉曼信号强度较弱，活细胞的成像仍然具有挑战性。共聚焦拉曼显微镜将在近红外激光的基础上发展起来。该系统可用于研究感染疟疾的红细胞和癌细胞，以及寻找亚细胞结构与其他生物物理参数之间的关系，如细胞内的折射率变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Confocal Raman microscopy is a powerful technique for chemical imaging mainly because of its ability to provide chemical composition with high spatial resolution. Raman mapping of a cell were done by researchers. However, imaging live cell is still challenging because of the weak Raman signal strength. Confocal Raman microscopy will be developed based on NIR laser. This system can be used to study malaria-infected RBC and cancer cells as well as to find a relation between sub cellular structure and other biophysical parameters such as refractive index variations inside of a cell.