DEVELOPMENT OF CONFOCAL RAMAN MICROSCOPE FOR LIVE CELL IMAGING

用于活细胞成像的共焦拉曼显微镜的开发

• 批准号: 8364154
• 负责人: JEON WOONG KANG
• 金额: $ 2.23万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364154
• 关键词: Biomedical Research; Cells; Cellular Structures; Chemicals; Development; Funding; Grant; Image; Lasers; Life; Malaria; Maps; Microscope; Microscopy; National Center for Research Resources; Principal Investigator; Refractive Indices; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Signal Transduction; Source; System; Techniques; United States National Institutes of Health; Variant; base; cancer cell; cellular imaging; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Confocal Raman microscopy is a powerful technique for chemical imaging mainly because of its ability to provide chemical composition with high spatial resolution. Raman mapping of a cell were done by researchers. However, imaging live cell is still challenging because of the weak Raman signal strength. Confocal Raman microscopy will be developed based on NIR laser. This system can be used to study malaria-infected RBC and cancer cells as well as to find a relation between sub cellular structure and other biophysical parameters such as refractive index variations inside of a cell.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 共焦拉曼显微镜是一种强大的化学成像技术，主要是因为它能够提供具有高空间分辨率的化学成分。细胞的拉曼图谱是由研究人员完成的。然而，由于拉曼信号强度较弱，活细胞成像仍然具有挑战性。共焦拉曼显微技术将在近红外激光的基础上发展。该系统可用于研究疟疾感染的红细胞和癌细胞，以及发现亚细胞结构和其他生物物理参数之间的关系，如细胞内的折射率变化。