GBV-B: A SMALL PRIMATE MODEL FOR HEPATITIS C INFECTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 丙型肝炎病毒感染影响全球约2%的人口。 该病毒具有诱导持续感染的高倾向，随着时间的推移，该感染进展为严重的肝脏疾病，包括肝硬化和肝癌。 由于缺乏小动物模型和组织培养系统，对HCV的研究一直受到阻碍。 GBV-B是在遗传学上与HCV最密切相关的病毒，并且它在绢毛猴中引起肝炎。 我们已经开发了GBV-B动物模型作为HCV的替代小灵长类动物模型。我们最近已经证明，普通的绒猴同样允许GBV-B感染，现在是首选的动物模型。 此外，我们已经开发了一个强大的组织培养系统GBV-B利用原代肝细胞培养的绢毛猴或绒猴。 在本提案中，我们将更全面地开发GBV-B的动物和培养模型，并测试与HCV疾病相关的特定假设。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hepatitis C virus infections affect approximately 2% of the worldwide population. The virus has a high propensity for inducing persistent infection that over time progress to serious liver disease including cirrhosis and liver cancer. Research on HCV has been impeded by the lack of a small animal model and a tissue culture system. GBV-B is the virus phylogenetically most closely related to HCV and it causes hepatitis in tamarins. We have developed the GBV-B animal model as a surrogate small primate model for HCV. We have recently demonstrated that the common marmoset is equally permissive for GBV-B infections and is now the preferred animal model. In addition, we have developed a robust tissue culture system for GBV-B utilizing primary hepatocyte cultures from tamarins or marmosets. In this proposal, we will more fully develop the animal and culture models for GBV-B and test specific hypotheses relevant to HCV disease.