A Data Coordinating Center for modENCODE

modENCODE 数据协调中心

• 批准号: 7825450
• 负责人: LINCOLN D. STEIN
• 金额: $ 132.94万
• 依托单位: ONTARIO INSTITUTE FOR CANCER RESEARCH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-04 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7825450
• 关键词: Age; Base Pairing; Binding Sites; Bioinformatics; Biological Process; Books; Caenorhabditis elegans; Chromatin; Code; Communities; Complex; Computers; Data; Data Coordinating Center; Data Files; Data Set; Databases; Deoxyribonuclease I; Development; Elements; Ensure; Eukaryota; Evolution; Familiarity; Genbank; Generic Drugs; Genes; Genome; Genomics; Goals; Human; Human Genome; Indium; Institutes; Knowledge; Mediating; Metadata; Methods; Modeling; Nucleotides; Pattern; Performance; Phase; Pilot Projects; Positioning Attribute; Procedures; Process; Production; Provider; Quality Control; RNA Splicing; Reading; Reagent; Reporting; Research; Research Personnel; Research Project Grants; Resources; Schedule; Scientist; Site; Sorting - Cell Movement; Source; Staging; Structure; System; Techniques; Testing; Tissues; Variant; Work; base; data mining; design; digital; experience; file format; fly; model organisms databases; open source; research study; software systems; technology development; tool

DESCRIPTION: The modENCODE project is a key sequel to the sequencing of the fly and worm genomes, and will have an enormous impact on our understanding of biological processes in all higher eukaryotes, including human. In order to manage the diverse, large-scale datasets that will be produced by modENCODE, we propose to create a data coordinating center (DCC) to track the data, integrate it with other information sources, and make it available to the research community in a timely and open fashion. This proposal brings together four groups with highly relevant backgrounds: The Micklem group, through its work on the InterMine system and FlyMine database, has extensive experience in integrating diverse types of data into high-performance data mining systems. The Stein and Lewis groups bring to the project an intimate familiarity with the C. elegans and D. melanogaster genomes, their reagents and research communities, and are well-positioned by their work with the WormBase and FlyBase databases to liaise with those MODs. The Kent group is responsible for the DCC for the Human ENCODE pilot project, and has extensive practical knowledge of developing and managing projects of this sort. We will assemble a team of three data managers stationed at CSHL and at Berkeley, who have a background in the bioinformatics of C. elegans and/or D. melanogaster. The managers will liaise with their contacts at the data provider sites to determine data file formats, milestones and quality control procedures for their datasets. They will also liaise with representatives from NCBI to coordinate modENCODE activities with the primary data repositories at GenBank and GEO. Data providers will upload their data sets to a staging server where they will be able to preview their data on an instance of the GBrowse genome browser. The data managers will QC the data before approving its transfer to the production database. Data will be integrated in the production database using InterMine, and from there released to the public on a monthly schedule. Researchers will be able to access the data via the GBrowse genome browser, bulk downloads, and via complex queries and reports mediated by InterMine and the BioMart data warehousing system. All major software systems used by the proposed DCC will be based on open source tools from the Generic Model Organism Database (GMOD), human ENCODE, and other sources. Throughout the project, Lewis and Stein will work close with FlyBase and/or WormBase to ensure that data collected by modENCODE becomes an integral part of the relevant model organism database. In addition we will dedicate a significant part of a data manager's effort to transfer data from modENCODE into the MODs during the last year of the project.

描述：Modencode项目是苍蝇和蠕虫基因组测序的关键续集，并且将对我们对包括人类在内的所有较高真核生物的生物过程的理解产生巨大影响。为了管理将由Modencode生产的多样化的大规模数据集，我们建议创建一个数据协调中心（DCC）以跟踪数据，将其集成到其他信息源，并及时且开放的方式向研究社区提供。该提案汇集了四个具有高度相关背景的小组：Micklem组通过其在Intermine系统和Flymine数据库中的工作，在将各种类型的数据集成到高性能数据挖掘系统中，具有丰富的经验。斯坦因和刘易斯团体将其与C. exemans和D. melanogaster基因组，其试剂和研究社区非常熟悉，并因其在Wormbase和Flybase数据库中的合作而妥善地与这些mod相处。肯特集团（Kent Group）负责人类编码试点项目的DCC，并且对开发和管理此类项目具有广泛的实践知识。我们将组建一个驻扎在CSHL和伯克利的三个数据经理组成的团队，他们在C. elegans和/或D. Melanogaster的生物信息学方面具有背景。管理人员将与数据提供商站点的联系人联系，以确定其数据集的数据文件格式，里程碑和质量控制程序。他们还将与NCBI的代表联系，以与GenBank和Geo的主要数据存储库进行协调调整活动。数据提供商将将其数据集上传到登台服务器，在那里他们能够在GBROWSE基因组浏览器的实例上预览数据。数据经理将在批准将其传输到生产数据库之前QC。数据将使用Intermine在生产数据库中集成，并按月向公众发布。研究人员将能够通过GBROWSE基因组浏览器，批量下载以及由Intermine和BiomArt数据仓库系统介导的复杂查询和报告访问数据。拟议的DCC使用的所有主要软件系统将基于来自通用模型有机体数据库（GMOD），人编码和其他来源的开源工具。在整个项目中，Lewis和Stein将与Flybase和/或Wormbase紧密合作，以确保Modencode收集的数据成为相关模型生物数据库的组成部分。此外，在项目的最后一年中，我们将专门将数据经理从Modencode传输到MOD的重要组成部分。