Immunology

免疫学

• 批准号: 8198155
• 负责人: R. PAUL JOHNSON
• 金额: $ 36.47万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8198155
• 关键词: AIDS Vaccines; Antibodies; Antibody Formation; Biological Assay; Blood; Cellular Immunity; Consensus; Consensus Development; Data; Data Analyses; Databases; Ensure; Enzyme-Linked Immunosorbent Assay; Evaluation; Flow Cytometry; Generations; Goals; Grant; HIV; Humoral Immunities; Immune; Immune response; Immunology; Immunophenotyping; Individual; Infection; Laboratories; Location; Measurement; Performance; Play; Protocols documentation; Reproducibility; Resources; SIV; Serum; Site; Specimen; Standardization; Subfamily lentivirinae; Testing; Vaccine Research; Vaccines; Vagina; Validation; Virus; antibody-dependent cell cytotoxicity; comparative; cytokine; improved; mucosal site; neutralizing antibody; nonhuman primate; prevent; rectal; response; simian human immunodeficiency virus; transmission process

The overall goals of the CAVR Immunology Core are to provide a centralized resource for analysis of SIV- and SHIV-specific humoral immune responses in serum and mucosal specimens and to standardize the performance of flow cytometric assays that will be carried out by individual laboratories. A comprehensive range of well-standardized and quantitative assays of humoral immunity will be offerered, including a panel of complementary assays for the determination of neutralizing antibody responses and a newly developed assay that provides sensitive and reproducible determination of ADCC activity. To ensure consistent generation of polychromatic flow cytometry data and assays of cell-mediated immunity, the Immunology Core will coordinate the generation of consensus standard operating protocols (SOPs) for immunophenotyping and cytokine flow cytometry (CFC) assays, and supervise the implementation and validation of each SOP at each location. Specific Aims include: 1. To provide a centralized resource for the analysis of SIV/SHlV-specific antibodies in sera using ELISA and SIV/SHIV neutralization assays; 2. To provide a centralized resource for analysis of SIV and SHIV-specific antibody responses in vaginal and rectal secretions; and 3. To ensure standardization of immunophenotyping and ICS assays conducted by different sites through the development of consensus protocols, implementation of proficiency testing, and comparative evaluation of flow cytometric data analysis. These activities will provide a comprehensive and robust assessment of host immune responses elicited by these vaccine approaches and will help define responses that are able to interrupt mucosal transmission of lentiviruses.

CAVR免疫学核心的总体目标是提供一个集中的资源，用于分析SIV-和 血清和粘膜标本中SHIV特异性体液免疫反应并使其标准化 将由各个实验室进行的流式细胞仪检测的性能。全面 将提供一系列标准化和定量的体液免疫测定，包括一组 用于确定中和抗体反应的补充测定法和新开发的 该测定提供灵敏且可再现的ADCC活性测定。以确保一致 多色流式细胞术数据的产生和细胞介导的免疫测定，免疫学 核心将协调生成共识标准操作方案（SOP）， 免疫表型和细胞因子流式细胞术（CFC）分析，并监督实施， 在每个地点验证每个SOP。具体目标包括：1。为了提供一个集中的资源， 使用ELISA和SIV/SHIV中和测定分析血清中的SIV/SHIV特异性抗体; 2.到 为分析阴道和直肠中SIV和SHIV特异性抗体反应提供集中资源 分泌物;和3.为了确保由不同机构进行的免疫表型和ICS测定的标准化， 通过制定共识协议、实施能力验证， 流式细胞术数据分析的比较评价。这些活动将提供一个全面和 对这些疫苗方法引起的宿主免疫应答进行了强有力的评估， 这些反应能够中断慢病毒的粘膜传播。