Immunology

免疫学

• 批准号: 8198155
• 负责人: R. PAUL JOHNSON
• 金额: $ 36.47万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8198155
• 关键词: AIDS Vaccines; Antibodies; Antibody Formation; Biological Assay; Blood; Cellular Immunity; Consensus; Consensus Development; Data; Data Analyses; Databases; Ensure; Enzyme-Linked Immunosorbent Assay; Evaluation; Flow Cytometry; Generations; Goals; Grant; HIV; Humoral Immunities; Immune; Immune response; Immunology; Immunophenotyping; Individual; Infection; Laboratories; Location; Measurement; Performance; Play; Protocols documentation; Reproducibility; Resources; SIV; Serum; Site; Specimen; Standardization; Subfamily lentivirinae; Testing; Vaccine Research; Vaccines; Vagina; Validation; Virus; antibody-dependent cell cytotoxicity; comparative; cytokine; improved; mucosal site; neutralizing antibody; nonhuman primate; prevent; rectal; response; simian human immunodeficiency virus; transmission process

The overall goals of the CAVR Immunology Core are to provide a centralized resource for analysis of SIV- and SHIV-specific humoral immune responses in serum and mucosal specimens and to standardize the performance of flow cytometric assays that will be carried out by individual laboratories. A comprehensive range of well-standardized and quantitative assays of humoral immunity will be offerered, including a panel of complementary assays for the determination of neutralizing antibody responses and a newly developed assay that provides sensitive and reproducible determination of ADCC activity. To ensure consistent generation of polychromatic flow cytometry data and assays of cell-mediated immunity, the Immunology Core will coordinate the generation of consensus standard operating protocols (SOPs) for immunophenotyping and cytokine flow cytometry (CFC) assays, and supervise the implementation and validation of each SOP at each location. Specific Aims include: 1. To provide a centralized resource for the analysis of SIV/SHlV-specific antibodies in sera using ELISA and SIV/SHIV neutralization assays; 2. To provide a centralized resource for analysis of SIV and SHIV-specific antibody responses in vaginal and rectal secretions; and 3. To ensure standardization of immunophenotyping and ICS assays conducted by different sites through the development of consensus protocols, implementation of proficiency testing, and comparative evaluation of flow cytometric data analysis. These activities will provide a comprehensive and robust assessment of host immune responses elicited by these vaccine approaches and will help define responses that are able to interrupt mucosal transmission of lentiviruses.

CAVR免疫学核心的总体目标是为SIV和SIV-和 血清和粘膜标本中的SHIV特异性体液免疫反应，并标准化 单个实验室将进行的流式细胞术测定的性能。全面 将提供一系列标准化和定量的体液免疫测定法，包括面板 确定中和抗体反应的互补测定法和新开发的 提供敏感且可重现的ADCC活性的测定。确保一致 多色流式细胞仪数据和细胞介导的免疫力的测定，免疫学 核心将协调共识标准操作协议（SOP）的生成 免疫表型和细胞因子流式细胞仪（CFC）测定法，并监督实施和 在每个位置验证每个SOP。具体目的包括：1。提供集中资源 使用ELISA和SIV/SHIV中和测定法分析SIV/SHLV特异性抗体； 2 提供集中资源来分析阴道和直肠的SIV和SHIV特异性抗体反应 分泌物；和3。确保不同的免疫表型和ICS分析的标准化 通过制定共识协议，实施能力测试以及 流式细胞仪数据分析的比较评估。这些活动将提供全面的 对这些疫苗方法引起的宿主免疫反应的强有力评估，并将有助于定义 能够中断慢病毒的粘膜传播的反应。