Analytical Core

由包括脂肪组织在内的内分泌器官产生的激素和由 免疫细胞和脂肪细胞在调节能量平衡和糖代谢方面起着重要作用。 组织脂和细胞内去脂代谢产物也调节代谢信号通路并影响 胰岛素抵抗。为了了解转基因小鼠代谢表型的潜在基础 改变新陈代谢，分析核心测量已知影响能量平衡的血清和组织因子 和葡萄糖动态平衡。分析核心的目标是提供标准化和高吞吐量 使用最先进的仪器对从以下来源获得的血清和组织样本进行分析测量 转基因小鼠。核心在大卫·哈伦博士作为核心董事的支持下运作，并 兰德尔·弗里德林博士、克劳斯·佩希霍尔德博士和斯科特·谢弗博士担任联席董事。分析核心提供了 以下服务：1)高通量和多路测量血清/组织/细胞培养水平 使用Luminex和Meso Scale Discovery多路复用技术的激素、细胞因子和趋化因子 2)使用Cobas高通量测量血液/组织/尿液中代谢物和电解质的水平 临床化学分析仪，3)与胰岛素相关的细胞内信号通路的分子分析 作用，葡萄糖代谢和炎症，4)细胞内脂质和 用三重四极杆质谱仪测定脂类代谢物，以及5)胰岛组织学评估 以及胰岛细胞亚群的基因转录分析。分析核心执行质量和 支持新陈代谢和心血管并发症核心的可重复测量 转基因小鼠的综合表型鉴定。

Hormones produced by endocrine organs including adipose tissue and inflammatory cytokines produced by immune cells and adipocytes play a major role in regulating energy balance and glucose metabolism. Tissue lipid and intracellular lipid-defived metabolites also modulate metabolic signaling pathways and affect insulin resistance. To understand the underlying basis of metabolic phenotypes of transgenic mice with altered metabolism, the Analytical Core measures serum and tissue factors known to affect energy balance and glucose homeostasis. The goal ofthe Analytical Core is to provide standardized and high-throughput analytical measurements using state-of-the-art instruments in serum and tissue samples obtained from transgenic mice. The Core operates under the supen/ision of Dr. David Harlan as the Core Director and Drs. Randall Friedline, Klaus Pechhold, and Scott Shaffer as co-Directors. The Analytical Core provides the following services: 1) High-throughput and multiplexed measurement of serum/tissue/cell culture levels of hormones, cytokines, and chemokines using Luminex and Meso Scale Discovery multiplexing technology, 2) High-throughput measurement of blood/tissue/urine levels of metabolites and electrolytes using a Cobas Clinical Chemistry Analyzer, 3) Molecular analysis of intracellular signaling pathways associated with insulin action, glucose metabolism, and inflammation, 4) Measurement of intracellular levels of lipids and lipid-derived metabolites using triple quadrupole mass spectrometry, and 5) Assessment of islet histology and gene transcriptional analysis of pancreatic islet cell subsets. The Analytical Core performs quality and reproducible measurements to support the Metabolism and Cardiovascular Complications Core for comprehensive phenotyping of transgenic mice.