Development and maintenance of the alveolar type 1 cell

这份新的调查员提案描述了一项为期5年的培训计划，以促进职业生涯的发展。 肺部医学方面的精神科科学家。首席调查员已经完成了在 在波士顿大学的肺部和重症监护医学和一年的研究奖学金 斯坦福大学生物化学系，现在继续在那里担任医学 医学部肺部和重症监护科。 在进行拟议研究时，首席调查员将获得肺组织学方面的专门知识， 小鼠遗传学、分子生物学和基因组学方法。马克·A·克拉斯诺博士将指导校长 侦查员的科学发展。他是斯坦福大学生物化学教授兼主席， 霍华德·休斯医学研究所的一名研究员，他训练了许多人 建立自己的实验室，并成功争取独立资金。《校长》 研究人员还征集了肺上皮细胞领域的三个国际公认的权威机构 生物学、肺泡发育和小鼠遗传学分别作为顾问。他将与 导师和顾问，以确保特定目标的实现。由资深人士组成的咨询委员会 斯坦福大学的调查人员也已经集结起来，将提供科学和职业建议。 研究重点将放在肺泡1型(AT1)细胞的发育和维持上。具体目标 包括：1)利用分子标记鉴定和鉴定AT-1细胞的双潜能前体细胞 并确定它在何时何地发生，以及它在肺发育过程中如何变化，2)使用 小鼠转基因技术标记和追踪成熟AT-2细胞的命运以确定AT-2是否 细胞是AT-1细胞的专性祖细胞，3)通过使用RNA识别AT-1命运的潜在调节因子 用原位杂交法筛选在末端空间选择性表达的转录因子基因 AT1形态发生时的上皮细胞。 通过汇集来自多个部门的独特资源，该计划整合了专门的 确保实现具体目标的专业领域。这项工作将与 了解并最终治疗肺气肿、肺癌和肺纤维化等肺部疾病。

This new investigator proposal describes a 5 year training program for the development of a career as a Dhysician scientist in pulmonary medicine. The principal investigator has completed a fellowship in pulmonary and critical care medicine at Boston University and a one year research fellowship in the Department of Biochemistry at Stanford University, where he now continues as an Instructor in Medicine the Pulmonary and Critical Care division of the Department of Medicine. In carrying out the proposed research, the Principal Investigator will acquire expertise in lung histology, mouse genetics, molecular biology, and genomic approaches. Dr. Mark A. Krasnow will mentor the Principal Investigator's scientific development. He is Professor and Chairman of Biochemistry at Stanford and an Investigator in the Howard Hughes Medical Institute who has trained numerous individuals who have gone on to establish their own laboratories and successfully compete for independent funding. The Principal Investigator has also enlisted three internationally recognized authorities in the fields of lung epithelial cell biology, alveolar development and mouse genetics, respectively, as consultants. He will work closely with the mentor and consultants to ensure fulfillment of the specific aims. An advisory committee of senior investigators at Stanford has also been assembled and will provide scientific and career advice. Research will focus on development and maintenance of the alveolar type 1 (AT 1) cell. Specific aims include: 1) Identify and characterize using molecular markers the putative bi-potential progenitor of AT 1 cells in mouse lung and determine when and where it arises, and how it changes during lung development, 2) Use mouse transgenic techniques to label and trace the fate of mature AT 2 cells to determine whether the AT 2 cell is an obligate progenitor to the AT 1 cell, 3) To identify potential regulators of AT 1 fate by using RNA in situ hybridization to screen for transcription factor genes that are selectively expressed in terminal airspace epithelium at the time of AT 1morphogenesis. By bringing together unique resources from multiple departments, this program integrates specialized areas of expertise that will ensure fulfillment of the specific aims. This work will be relevant for understanding and ultimately treating lung diseases like emphysema, lung cancer, and pulmonary fibrosis.