Brain Imaging In Human Aging, Alzheimer Disease And Other Disorders

人类衰老、阿尔茨海默病和其他疾病的脑成像

• 批准号: 8335771
• 负责人: Stanley I. Rapoport
• 金额: $ 17.24万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8335771
• 关键词: Acids; Adult; Affect; Age; Aging; Agonist; Alzheimer' s Disease; Alzheimer' s disease risk; Amyloid; Animal Model; Anterior; Anti-Inflammatory Agents; Anti-inflammatory; Apomorphine; Arachidonic Acids; Area; Atrophic; Behavior; Bilateral; Biological Markers; Biological Preservation; Blood flow; Brain; Brain imaging; Brain region; Bypass; Cerebellum; Cerebrospinal Fluid; Cerebrovascular Circulation; Clinical; Clinical Protocols; Cognition; Collaborations; Consumption; Corpus striatum structure; Data; Defect; Dementia; Disease; Docosahexaenoic Acids; Dopamine; Dopamine D2 Receptor; Drug Design; Drug effect disorder; Early identification; Event; Evolution; Family history of; Fatty Acids; Functional disorder; Glucose; Glycolysis; Goals; Grant; HIV-1; Hepatic; Hominidae; Human; Image; Impaired cognition; Infection; Institutional Review Boards; Insula of Reil; Label; Magnetic Resonance Imaging; Measurable; Measures; Medial; Medicine; Mental Depression; Metabolic; Metabolism; Methods; Mitochondria; Modeling; National Institute of Allergy and Infectious Disease; Nausea; Neocortex; Nerve Degeneration; Neurofibrillary Tangles; Neuronal Plasticity; Neurons; Parkinson Disease; Pathologic Processes; Pathology; Pathway Analysis; Patients; Pattern; Phospholipase A2; Plasma; Positron; Positron-Emission Tomography; Process; Protocols documentation; Psychiatry; Publications; Quantitative Autoradiography; RNA; Radiochemistry; Radiolabeled; Rattus; Receptor Activation; Relative (related person); Reporting; Research Personnel; Rodent; Role; Saline; Schizophrenia; Senile Plaques; Serum; Severities; Signal Transduction; Somatotropin; Structure; Surrogate Markers; Synapses; Testing; Thalamic structure; Transgenic Organisms; Translating; United States National Institutes of Health; Viral; Work; age related; aging brain; alpha-Linolenic Acid; antiretroviral therapy; apolipoprotein E-4; base; brain metabolism; brain volume; cytokine; disease diagnosis; excitotoxicity; frontal lobe; glucose metabolism; glucose transport; gray matter; healthy aging; human disease; imaging modality; improved; in vivo; inflammatory marker; insulin sensitivity; medical schools; men; neurochemistry; neuroinflammation; neurotransmission; novel; pathological aging; preclinical study; prevent; putamen; radiotracer; receptor coupling; response; tau Proteins; tau-1; trimethobenzamide; uptake; white matter

BRAIN VOLUME NETWORK CHANGES WITH HEALTHY AGING Healthy aging is associated with volume reductions in the frontal cortex and other brain regions. We developed a Scaled Subprofile Model (SSM) to identify brain network changes in relation to age in healthy adults, using volume measured obtained with magnetic resonance imaging (MRI). The SSM analysis identified a regional pattern of gray matter atrophy associated with healthy aging that included reductions in bilateral dorsolateral and medial frontal, anterior cingulate, insula/perisylvian, precuneus, parietotemporal, and caudate regions with areas of relative preservation in bilateral cerebellum, thalamus, putamen, mid cingulate, and temporal pole regions. Network analysis with SSM can help detect reproducible age-related MRI patterns, assisting efforts in the study of healthy and pathological aging (Bergfield et al., 2010). IMAGING HUMAN BRAIN SIGNALING INVOLVING DOPAMINE. We have developed a novel method to image dopaminergic neurotransmission in the human brain using positron emission tomography (PET) and 1-11Carachidonic acid (1-11CAA), as well as regional cerebral blood flow (rCBF) with 15Owater. We measured regional incorporation coefficients K* for AA and rCBF in healthy men given the dopaminergic D1/D2 receptor agonist (10 or 20 mg/kg s.c.) apomorphine or saline, after pretreatment with trimethobenzamide to prevent nausea. We confirmed a robust central dopaminergic response to apomorphine by observing significant increases in serum concentration of growth hormone. We observed significant increases as well as decreases in K* and increments in rCBF in response to apomorphine. The changes in AA incorporation likely reflect neuronal signaling events downstream of activated D2-like receptors coupled to phospholipase A2 within the striatum. The changes in rCBF are consistent demonstrate net functional effects of D1/D2 receptor activation. Based on these data, the 1-11CAA PET method should be useful for studying disturbances of dopaminergic neurotransmission in conditions such as Parkinson disease and schizophrenia. Results of this work have been submitted for publication. IMAGING NEUROINFLAMMATION IN ALZHEIMER DISEASE An in vivo imaging method to evaluate neuroinflammation in Alzheimer disease patients could help in the early identification of neuroinflammation, in understanding its contribution to dementia status, and in evaluating efficacy of antiinflammatory and other agents. We used our in vivo imaging method to identify increased uptake of intravenously infused radiolabeled arachidonic acid into the brain as a marker of inflammation in a rat model. Based on this evidence, we then used positron emission tomography (PET) to demonstrate increased brain arachidonic uptake as a marker of neuroinflammation in patients with Alzheimer disease (Esposito et al., J Nucl Medicine. 2008 49:1414-21). Based on this work, in collaboration with researchers at the Departments of Psychiatry at NYU School of Medicine and of Radiochemistry at Weill Cornell Medical College, under an NIH grant we are imaging neuroinflammation and brain glucose metabolism with PET in Alzheimer disease patients in relation to dementia severity and amyloid distribution. IMAGING NEUROINFLAMMATION IN HIV-1 INFECTED SUBJECTS Thirty million people worldwide are infected with the Human Immunodeficiency Virus (HIV)-1; some develop dementia despite antiretroviral therapy, whereas up to 50% develop depression and some cognitive dysfunction. We hypothesized that cognitive dysfunction in HIV-1 patients is exacerbated by concurrent neuroinflammation, and that drugs designed to treat the neuroinflammation would reduce the dysfunction. To test this hypothesis, we first confirmed neuroinflammation as upregulated brain arachidonic acid metabolism in a noninfectious transgenic HIV-1 rat model, using our in vivo fatty acid imaging method (Basselin et al. Imaging upregulated brain arachidonic acid metabolism in HIV-1 transgenic rats. J Cereb Blood Flow Metab Jul 28 2010). On this basis, we now are preparing a collaborative clinical protocol supported by the NIAID to quantify brain arachidonic acid metabolism and regional cerebral blood flow with positron emission tomography (PET) in HIV-1 infected patients in relation to dementia severity and cerebrospinal fluid viral and cytokine load. This study should help to identify neuroinflammation in the course of HIV-1 infection, and to establish a surrogate marker for efficacy of anti-inflammatory therapy. DOCOSAHEXAENOIC ACID (DHA) INCORPORATION AS BIOMARKER OF BRAIN DHA METABOLISM AND NEUROTRANSMISSION. Docosahexaenoic acid (DHA) is critical for maintaining normal brain structure and function, and is considered neuroprotective. Its brain concentration depends on dietary DHA content and hepatic conversion from its dietary derived n-3 precursor, alpha-linolenic acid. We developed an in vivo method in rats using quantitative autoradiography and intravenously injected radiolabeled DHA to incorporation into brain of unesterified plasma DHA, and showed that the incorporation rate equals the rate of brain metabolic DHA consumption. We extended the method for use in humans with positron emission tomography (PET). BIOMARKERS AND EVOLUTION IN ALZHEIMER DISEASE. Brain regions and their highly neuroplastic long axonal connections, which expanded rapidly during hominid evolution, are preferentially affected by Alzheimer disease (AD), and there is no natural animal model with full disease pathology (neurofibrillary tangles and neuritic amyloid plaques). Thus, it is possible that AD is uniquely human disease that was introduced during hominid evolution. This introduction may be related to increased neuroplasticity of vulnerable regions. Measuring biomarkers such as reduced glucose metabolism in association neocortex and changes in arachidonic acid metabolism with PET, defects in long white matter tracts, RNA neurochemical changes, and high CSF levels of total and phosphorylated tau protein, may be useful to identify MCI and presymptomatic patients. These same biomarkers may help explain why humans appear to be uniquely vulnerable to this disease (Rapoport and Nelson, 2011). BRAIN FUEL METABOLISM, AGING, AND ALZHEIMER DISEASE. Lower brain glucose metabolism is present before the onset of measurable cognitive decline in people at risk for Alzheimer disease--carriers of apolipoprotein E4, and those with maternal family history of AD. These reports suggest that hypometabolism precedes and contribute to the neuropathological cascade leading to cognitive decline in AD. Reasons may include defects in brain glucose transport, disrupted glycolysis, or impaired mitochondrial function. A contributing role of deteriorating glucose availability to or metabolism by the brain in AD does not exclude the opposite effect, i.e., that neurodegenerative processes in AD further decrease brain glucose metabolism because of reduced synaptic functionality and hence reduced energy needs, thereby completing a vicious cycle. Strategies to reduce the risk of AD by breaking this cycle should aim to (1) improve insulin sensitivity by improving systemic glucose utilization, or (2) bypass deteriorating brain glucose metabolism using approaches that safely induce mild, sustainable ketonemia (Cunnane et al., 2011).

脑容量网络随健康衰老而变化