Enhancement of the Integrated Aquatic Biomedical Models Facility Core

描述（由申请人提供）：我们申请资金用于俄勒冈州立大学Sinnhuber水生研究实验室（SARL）的电力服务现代化，应急电力和更换饮食准备厨房。17000平方英尺。SARL建于46年前，提供了世界上其他地方无法提供的能力，以支持水生脊椎动物模型的生物医学研究和培训。水生脊椎动物人类生物学模型正迅速成为生物医学研究的一个快速、经济、强大的研究平台。在其显著的研究成就中，SARL对完成两个最大的在任何脊椎动物物种（约42,000鳟鱼）中进行的超低剂量致癌性研究至关重要，并帮助确定了alfatoxin是一种主要的人类致癌物。该设施最近获得了NIH RC4主任的拨款，用于进一步开发新的机器人和自动化技术，使斑马鱼成为第一个能够进行高通量和高含量筛选的脊椎动物模型生物。SARL最近获得的其他设备，包括自动化液体处理、机器人样品处理和分析以及高含量成像仪器，大大增加了研究纳米材料、药物和其他环境化学物质对斑马鱼的药理学、安全性和毒理学影响的能力。SARL已经取得了许多里程碑，使其处于斑马鱼研究的前沿。其中包括：“第一个也是唯一一个特定的无病原体斑马鱼设施”，第一个为斑马鱼定义的饮食，“标准化的近亲繁殖SPF”野生型“斑马鱼”，“自动化繁殖和斑马鱼处理的多项创新技术”。SARL目前拥有24名研究人员和工作人员，并在2010年支持520万美元的联邦研究直接费用。SARL设施还支持许多学生、博士后和兽医在使用水生模型方面的培训。两项T32培训资助是基于SARL设施的独特能力：一项新的兽医培训资助（DVM）获得博士学位，以及俄勒冈州立大学36年的T32毒理学培训资助。所申请的资金将用于完成一项为期7年的改造计划，以重建一个核心设施，以支持有史以来规模最大的脊椎动物实验。所要求的应急电力系统和新的电力服务对于保护鱼类健康和防止长期实验中断是必要的。饮食厨房的改造是必要的，以保护设施中饲养的大量动物的食物供应，并允许在现场准备定制的饮食。这将增加SARL的独特能力，以探索斑马鱼营养缺乏的影响。

DESCRIPTION (provided by applicant): We request funds to modernize the electric service, the emergency electrical power, and replace the diet preparation kitchen at the Sinnhuber Aquatic Research Laboratory (SARL) at Oregon State University. The 17,000 sq. ft. SARL was built 46 years ago and provides capabilities found nowhere else in the world to support biomedical research and training in aquatic vertebrate models. Aquatic vertebrate models of human biology are rapidly emerging as a rapid, cost effective, and powerful research platform for biomedical studies. Among its notable research accomplishments, the SARL was essential for completing the two largest ultralow dosage carcinogenesis studies ever conducted in any vertebrate species (~42,000 trout) and helped identify alfatoxin as a major human carcinogen. The Facility has recently received an NIH RC4 Director's grant to further develop new robotics and automation to enable zebrafish to become the first vertebrate model organism capable of high throughput and high-content screening. Other equipment recently acquired for SARL, including automated liquid handling, robotic sample processing and analysis, and high content imaging instrumentation, has greatly added to the capacity for studying the pharmacologic, safety and toxicological effects of nanomaterials, drugs, and other environmental chemicals in zebrafish. SARL has achieved a number of milestones to place it in the forefront of zebrafish research. These include: "the first and only specific pathogen-free zebrafish facility," the first defined diet for zebrafish, "a standardized outbred SPF "wild-type" zebrafish," multiple innovative technologies to automate breeding and zebrafish handling. SARL currently houses 24 researchers plus staff and supports $5.2 million in direct costs of federal research in 2010. The SARL facility also supports the training of many students, post- docs and veterinarians in the use of aquatic models. Two T32 training grants are based upon the unique capabilities of the SARL facility: a new training grant for veterinarians (DVM) to earn the Ph.D., and the 36-year T32 Toxicology Training grant at OSU. The requested funds will enable the completion of a seven-year remodeling program to rebuild a core facility will supporting the largest vertebrate experiments ever conducted. The requested emergency power system and new electrical service is necessary to protect fish health and to prevent disruption of long-term experiments. The diet kitchen remodeling is necessary to protect the food supply for the large numbers of animals maintained in the facility as well as to allow custom-defined diets to be prepared on site. This will add to SARL's unique capabilities to probe the effects of nutritional deficiencies to be investigated in zebrafish.