Intrinsic and synaptic determinants of activity in GPe neurons in PD models

PD 模型中 GPe 神经元活性的内在和突触决定因素

• 批准号: 8544579
• 负责人: DALTON JAMES SURMEIER
• 金额: $ 11.59万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8544579
• 关键词: Address; Animal Model; Basal Ganglia; Behavior; Bradykinesia; Brain; Brain region; Cell Nucleus; Cessation of life; Coupling; Deep Brain Stimulation; Development; Disease; Disease model; Dopamine; Down-Regulation; Economics; Ensure; Equilibrium; Frequencies; Functional disorder; Globus Pallidus; Glutamates; Grant; Human; Ion Channel; Laboratories; Lesion; Link; Modeling; Molecular; Monkeys; Motor; Neurodegenerative Disorders; Neurons; Parkinson Disease; Pathology; Patients; Pattern; Periodicity; Pharmacological Treatment; Primates; Property; Rodent; Rodent Model; Role; Signal Transduction; Staging; Structure of subthalamic nucleus; Substantia nigra structure; Symptoms; Synapses; Testing; Theoretical Studies; Therapeutic; Translations; Tremor; Viral; Work; dopaminergic neuron; gene therapy; insight; novel; novel therapeutics; pars compacta; restoration; skills

Parkinson's disease (PD) is the second most common neurodegenerative disease in the U.S. The core motor symptoms of PD are attributable to the degeneration of the mesencephalic dopaminergic neurons and alterations in the activity of neurons in the basal ganglia. In PD patients and in primate PD models, neurons in two key nuclei of the basal ganglia - the external segment of the globus (GPe) and the subthalamic nucleus (STN) - spike in synchronous, high frequency rhythmic bursts.This pathophysiological activity is thought to be responsible for bradykinesia, akinesia and rigidity in PD patients.Theoretical studies suggest that autonomous pacemaking in GPe neurons counter-balances the natural tendency of the reciprocally connected, STN-GPe network to transition into the pathological synchronous, rhythmic bursting seen in PD. The model that has dominated the field for the last two decades has assumed that following DA depletion there is an elevation in striatopallidal GABAergic inhibitory input to the GPe, leading to a suppression of this autonomous activity. In the course of pursuing this hypothesis, we discovered that DA depletion induces a change in the intrinsic properties of GPe neurons that results in the loss of autonomous pacemaking. Moverover, this loss appears to be attributable to the down-regulation of a single ion channel subunit (HCN2). It is our central hypothesis that the loss of autonomous pacemaking is responsible for the emergence of synchronous rhythmic bursting of the STN-GPe network in PD and that reversing this adaptation will not only diminish the pathophysiology in this network, it will alleviate the motor symptoms ofPD. This project blends the skills of the labs of Drs. Surmeier, Wilson, Kita and Osten to pursue four specific aims addressing the basic mechanisms underlying this 'silencing' in rodent and monkey models of PD as well as strategies that could be used in PD patients to correct the deficit. Our aims are: 1) to characterize the mechanisms governing the rate and regularity of autonomous pacemaking in GPe neurons and their adaptation in rodent PD models (Wilson); 2) to characterize the mechanisms governing the suppression of pacemaking in GPe neurons in rodent PD models and to develop a means for its restoration (Surmeier, Osten, Kita); 3) to characterize subthalamo-pallidal glutamatergic signaling in rodent PD models and its potential role in suppression of pacemaking (Surmeier); 4) to characterize the role of subthalamo-pallidal synaptic signaling in controlling GPe activity and its adaptations in a monkey model of PD (Kita). Lay summary: These studies are aimed at correcting dysfunctional brain activity in late stage PD. The successful attainment of our aims could not only provide a novel, gene therapy for late stage PD but open new avenues for pharmacological treatment.

帕金森氏症（PD）是美国第二常见的神经退行性疾病