Interdepartmental Two-photon Imaging Center

跨部门双光子成像中心

• 批准号: 7816800
• 负责人: DALTON JAMES SURMEIER
• 金额: $ 59.62万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7816800
• 关键词: Interdepartmental; Two; photon; Imaging; Center

Cellular and molecular imaging technologies are revolutionizing neuroscience. The technology with the biggest potential impact on this field is "non-linear optical microscopy" which enables two-photon (2P) imaging of fluorescent structures deep in living tissue with unprecedented spatial and temporal resolution. Combining 2P imaging with electrophysiology and molecular uncaging creates an extraordinarily powerful tool that is having a profound effect on the conduct of neuroscientific study. The strategic goal of this revised proposal is to put this critical technology in the hands of a two highly productive groups of neuroscientists at Northwestern University (NU) that receive nearly $8M/year (direct costs) in funding from NINDS. One of these groups is focused on the properties of neuronal dendrites in health, aging and disease; the other group is focused on neural stem cell biology and its application to neuroregeneration. To maximize the investment of NU and NINDS in these research programs, both groups need ready access to user-friendly, multifunctional 2P imaging workstations. The following specific aims are designed to achieve this goal: 1. to further characterize and optimize the performance of our existing 2P imaging workstations; 2. to extend the capabilities of the existing workstations by adding new hardware and software features integrating electrophysiology and molecular uncaging; 3. to create additional core facilities with 2P imaging workstations and technical support for NIH funded investigators who do not currently have access to these resources; 4. to establish an infrastructure that ensures the safe, efficient and productive operation of all the cores; 5. to create an Internet-based distribution point for dissemination of information about applications, software and hardware design/implementation A corporate partner with expertise in the production of 2P workstations, Prairie Technologies, has been recruited to help achieve these aims. By establishing a dialogue between industry and researchers, we hope to accelerate the development of hardware and software that best meets the needs of the neuroscience community. The attainment of these aims will not only have a transforming impact on the NIH funded research programs at NU but it will significantly accelerate the delivery of these technologies to the broader neuroscience community, quicken the pace of scientific discovery and promote the development of new treatments for neurological disorders.

细胞和分子成像技术正在彻底改变神经科学。在这一领域具有最大潜在影响的技术是“非线性光学显微镜”，它能够以前所未有的空间和时间分辨率对活体组织深处的荧光结构进行双光子成像。将2P成像与电生理学和分子解锁相结合，创造了一种非常强大的工具，对神经科学研究的开展产生了深远的影响。这项修订提案的战略目标是将这项关键技术交给西北大学（NU）的两个高效神经科学家小组，他们每年从NINDS获得近800万美元（直接费用）的资助。其中一个小组专注于研究健康、衰老和疾病中神经元树突的特性；另一个小组主要研究神经干细胞生物学及其在神经再生中的应用。