Interdepartmental Two-photon Imaging Center

跨部门双光子成像中心

• 批准号: 8145904
• 负责人: DALTON JAMES SURMEIER
• 金额: $ 35.04万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8145904
• 关键词: Aging; Communities; Computer software; Core Facility; Dendrites; Development; Direct Costs; Disease; Electrophysiology (science); Ensure; Funding; Goals; Hand; Health; Image; Imaging technology; Industry; Information Dissemination; Internet; Investments; Life; Microscopy; Molecular; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Optics; Performance; Production; Property; Recruitment Activity; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Structure; Technology; Tissues; United States National Institutes of Health; Universities; base; design; meetings; molecular imaging; nerve stem cell; nervous system disorder; operation; programs; stem cell biology; tool; two-photon; user-friendly

Cellular and molecular imaging technologies are revolutionizing neuroscience. The technology with the biggest potential impact on this field is "non-linear optical microscopy" which enables two-photon (2P) imaging of fluorescent structures deep in living tissue with unprecedented spatial and temporal resolution. Combining 2P imaging with electrophysiology and molecular uncaging creates an extraordinarily powerful tool that is having a profound effect on the conduct of neuroscientific study. The strategic goal of this revised proposal is to put this critical technology in the hands of a two highly productive groups of neuroscientists at Northwestern University (NU) that receive nearly $8M/year (direct costs) in funding from NINDS. One of these groups is focused on the properties of neuronal dendrites in health, aging and disease; the other group is focused on neural stem cell biology and its application to neuroregeneration. To maximize the investment of NU and NINDS in these research programs, both groups need ready access to user-friendly, multifunctional 2P imaging workstations. The following specific aims are designed to achieve this goal: 1. to further characterize and optimize the performance of our existing 2P imaging workstations; 2. to extend the capabilities of the existing workstations by adding new hardware and software features integrating electrophysiology and molecular uncaging; 3. to create additional core facilities with 2P imaging workstations and technical support for NIH funded investigators who do not currently have access to these resources; 4. to establish an infrastructure that ensures the safe, efficient and productive operation of all the cores; 5. to create an Internet-based distribution point for dissemination of information about applications, software and hardware design/implementation A corporate partner with expertise in the production of 2P workstations, Prairie Technologies, has been recruited to help achieve these aims. By establishing a dialogue between industry and researchers, we hope to accelerate the development of hardware and software that best meets the needs of the neuroscience community. The attainment of these aims will not only have a transforming impact on the NIH funded research programs at NU but it will significantly accelerate the delivery of these technologies to the broader neuroscience community, quicken the pace of scientific discovery and promote the development of new treatments for neurological disorders.

细胞和分子成像技术正在彻底改变神经科学。对这一领域具有最大潜在影响的技术是“非线性光学显微镜”，它能够以前所未有的空间和时间分辨率对活组织深处的荧光结构进行双光子（2 P）成像。将2 P成像与电生理学和分子撑开相结合，创造了一个非常强大的工具，对神经科学研究的进行产生了深远的影响。这项修订提案的战略目标是将这项关键技术交给西北大学（NU）两个高产的神经科学家小组，他们每年从NINDS获得近800万美元（直接成本）的资金。其中一组专注于健康，衰老和疾病中神经元树突的特性;另一组专注于神经干细胞生物学及其在神经再生中的应用。 为了使NU和NINDS在这些研究项目中的投资最大化，这两个小组都需要随时访问用户友好的多功能2 P成像工作站。为实现这一目标，制定了以下具体目标： 1.进一步表征和优化我们现有的2 P成像工作站的性能; 2.通过增加新的硬件和软件功能来扩展现有工作站的功能，将电生理学和分子释放结合起来; 3.建立额外的核心设施，配备2 P成像工作站，并为目前无法获得这些资源的NIH资助的研究人员提供技术支持; 4.建立基础设施，确保所有核心的安全、高效和生产性运作; 5.创建一个基于互联网的分发点，用于传播有关应用程序、软件和硬件设计/实施的信息。为了帮助实现这些目标，聘请了一家在生产2 P工作站方面具有专业知识的公司合作伙伴Prairie Technologies。通过在工业界和研究人员之间建立对话，我们希望加快硬件和软件的开发，以最好地满足神经科学界的需求。 这些目标的实现不仅将对NIH资助的研究项目产生变革性影响，而且将大大加快这些技术向更广泛的神经科学界的交付，加快科学发现的步伐，促进神经系统疾病新疗法的发展。