Administrative/Statistics/Imaging Core

行政/统计/成像核心

基本信息

批准号：
8375082
负责人：
Hans Schreiber
金额：
$ 11.14万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-06-25 至 2014-05-31
项目状态：
已结题

项目摘要

The Administrative Part of Core A provides Dr. Schreiber protected time for scientific leadership and careful guidance of the Program Project. This leadership will be essential for achieving the goals of the program project that depend on integrating scientists with highly complementary but fundamentally different skills and operation modes and very diverse methodology and technology. Effective productive integration will depend on minimizing negative effects of the physical separation of the interacting scientists. This requires dedicated time from Dr. Schreiber for careful planning and using methods of easy interaction over long distances. For coordinating the scientific and technical activities of this program project, the Core will (i) organize a monthly steering committee meeting and research-in-progress meeting with the leaders of the four projects, the statistician and the core directors with careful planning of the agenda including distribution of data, manuscripts and papers to be discussed, (ii) organizes a more comprehensive quarterly meeting of all scientific and technical personnel involved in the program, (iii) prepare, distribute and maintain electronic files of reports, protocols and images and distribute relevant research articles, (iv) prepare and update existing databases for cell lines, gene constructs, monoclonal antibodies and vectors, and (v) monitor day-today expenses, support and organize the visits of one external scientific advisor and one external collaborator per year. The Statistics Part of Core A, provides a protected time to our statistician Dr. Karrison. This provides researchers with a highly experienced scholarly statistician who is dedicated to the success of this program project. This arrangement will give us much improved access to biostatistical consultation during the planning and execution and analysis stages of the proposed studies. The Imaging Part of Core A under the leadership of Dr. P. Charles Lin provides researchers with continued access to the first rate imaging institute at Vanderbilt for optical imaging using window chamber technology. The migration and localization of the fluorescent T cells or fluorochrome-tagged molecules to large well-established tumors will be monitored through a window opening on one side of these tumors using the Zeiss multiphoton laser scanning microscope LSM 510 META. The new META (Zeiss) spectral detectors resolve emissions from these different fluorescent dyes efficiently and exactly by providing linear spectral unmixing using specialized software. The microscopic imaging of the tumor microenvironment will give us detailed information on real time events and precise locality of cells or molecules injected into mice in the various models proposed.

核心A的行政部分为Schreiber博士提供了科学领导和谨慎的时间计划项目的指导。该领导对于实现计划的目标至关重要依靠将科学家与高度互补但根本不同的技能融合的项目和操作模式以及非常多样化的方法和技术。有效的生产融合将取决于最小化相互作用科学家的物理分离的负面影响。这需要 Schreiber博士的专用时间仔细计划和使用简易互动的方法距离。为了协调该计划项目的科学和技术活动，核心意愿（i）组织每月指导委员会会议和进行研究的会议四个项目，即统计学家和核心董事，仔细规划议程，包括分发（ii）组织的数据，手稿和论文，组织更全面的季度会议该计划涉及的所有科学和技术人员，（iii）准备，分发和维护电子报告，协议和图像的文件并分发相关的研究文章，（iv）准备和更新现有的细胞系，基因构建体，单克隆抗体和向量的数据库，以及（v）监视日期费用，支持和组织一位外部科学顾问和一位外部合作者的访问每年。 Core A的统计部分为我们的统计学家Karrison博士提供了受保护的时间。这为研究人员提供一位经验丰富的学术统计学家，他致力于成功计划项目。这种安排将为我们提供大大改善的生物统计咨询的机会拟议研究的计划，执行和分析阶段。核心A的成像部分 P. Charles Lin博士的领导使研究人员继续访问一流成像范德比尔特（Vanderbilt）的研究所使用窗室技术进行光学成像。迁移和本地化荧光T细胞或荧光素标记的分子到大型良好的肿瘤将是使用Zeiss多光子激光通过这些肿瘤的一侧的窗户开口进行监测扫描显微镜LSM 510 meta。新的元（Zeiss）光谱探测器解决了从这些不同的荧光染料有效，通过使用专业的线性光谱构想软件。肿瘤微环境的微观成像将为我们提供有关真实的详细信息在提出的各种模型中，时间事件和细胞或分子的精确位置注入小鼠。