Administrative/Statistics/Imaging Core

行政/统计/成像核心

• 批准号: 8270394
• 负责人: Hans Schreiber
• 金额: $ 11.53万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-25 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8270394
• 关键词: CD8B1 gene; Cell Line; Cells; Computer software; Consultations; Data; Databases; Electronics; Event; Fluorescent Dyes; Fluorochrome; Genes; Goals; Human Resources; Image; Improve Access; Institutes; Lasers; Leadership; Manuscripts; Methodology; Methods; Microscope; Microscopic; Modeling; Monitor; Monoclonal Antibodies; Mus; Paper; Protocols documentation; Reporting; Research; Research Personnel; Scanning; Scientist; Side; Staging; T-Lymphocyte; Technology; Time; Update; Visit; detector; experience; meetings; migration; operation; optical imaging; physical separation; programs; skills; statistics; success; tumor; vector

The Administrative Part of Core A provides Dr. Schreiber protected time for scientific leadership and careful guidance of the Program Project. This leadership will be essential for achieving the goals of the program project that depend on integrating scientists with highly complementary but fundamentally different skills and operation modes and very diverse methodology and technology. Effective productive integration will depend on minimizing negative effects of the physical separation of the interacting scientists. This requires dedicated time from Dr. Schreiber for careful planning and using methods of easy interaction over long distances. For coordinating the scientific and technical activities of this program project, the Core will (i) organize a monthly steering committee meeting and research-in-progress meeting with the leaders of the four projects, the statistician and the core directors with careful planning of the agenda including distribution of data, manuscripts and papers to be discussed, (ii) organizes a more comprehensive quarterly meeting of all scientific and technical personnel involved in the program, (iii) prepare, distribute and maintain electronic files of reports, protocols and images and distribute relevant research articles, (iv) prepare and update existing databases for cell lines, gene constructs, monoclonal antibodies and vectors, and (v) monitor day-today expenses, support and organize the visits of one external scientific advisor and one external collaborator per year. The Statistics Part of Core A, provides a protected time to our statistician Dr. Karrison. This provides researchers with a highly experienced scholarly statistician who is dedicated to the success of this program project. This arrangement will give us much improved access to biostatistical consultation during the planning and execution and analysis stages of the proposed studies. The Imaging Part of Core A under the leadership of Dr. P. Charles Lin provides researchers with continued access to the first rate imaging institute at Vanderbilt for optical imaging using window chamber technology. The migration and localization of the fluorescent T cells or fluorochrome-tagged molecules to large well-established tumors will be monitored through a window opening on one side of these tumors using the Zeiss multiphoton laser scanning microscope LSM 510 META. The new META (Zeiss) spectral detectors resolve emissions from these different fluorescent dyes efficiently and exactly by providing linear spectral unmixing using specialized software. The microscopic imaging of the tumor microenvironment will give us detailed information on real time events and precise locality of cells or molecules injected into mice in the various models proposed.

核心 A 的管理部分为 Schreiber 博士提供了科学领导和谨慎的受保护时间 计划项目的指导。这种领导力对于实现该计划的目标至关重要 该项目依赖于将具有高度互补但根本不同的技能的科学家整合在一起 操作模式以及非常多样化的方法和技术。有效的生产整合将取决于 最大限度地减少相互作用的科学家之间的物理分离的负面影响。这需要 Schreiber 博士投入大量时间进行仔细规划并使用长期轻松互动的方法 距离。为了协调本计划项目的科学和技术活动，核心将 (i) 每月组织一次指导委员会会议和与各机构领导人的研究进展会议 四个项目、统计员和核心主任仔细规划议程，包括分配 待讨论的数据、手稿和论文，(ii) 组织一次更全面的季度会议 参与该计划的所有科学和技术人员，(iii) 准备、分发和维护电子版 报告、方案和图像文件并分发相关研究文章，(iv) 准备和更新 细胞系、基因构建体、单克隆抗体和载体的现有数据库，以及 (v) 日常监测 费用，支持和组织一名外部科学顾问和一名外部合作者的访问 每年。核心 A 的统计部分为我们的统计学家 Karrison 博士提供了受保护的时间。这 为研究人员提供了一位经验丰富的学术统计学家，他致力于这项研究的成功 计划项目。这一安排将使我们在 拟议研究的规划、执行和分析阶段。下核心A的成像部分 P. Charles Lin 博士的领导为研究人员提供了持续获得一流成像的机会 范德比尔特研究所使用窗室技术进行光学成像。迁移和本地化 荧光 T 细胞或荧光染料标记分子对大型成熟肿瘤的影响将是 使用蔡司多光子激光器通过这些肿瘤一侧的窗口进行监测 扫描显微镜 LSM 510 META。新型 META（蔡司）光谱检测器可解决以下问题的排放： 通过使用专门的线性光谱分解技术，可以高效、准确地分离这些不同的荧光染料 软件。肿瘤微环境的显微成像将为我们提供真实的详细信息 在提出的各种模型中，时间事件和注射到小鼠体内的细胞或分子的精确位置。