Three Laser BD Digital LSR

描述（由申请人提供）：流式细胞术已成为许多生物和医学研究领域不可或缺的工具。可以在单个活细胞上同时测量多个参数，其速度可以在几秒到几分钟内分析整个细胞群。罕见和不寻常的事件可以快速识别和定义的细胞表面或细胞内分子的表达水平可以非常容易和精确地量化。检测此类事件的能力对于癌症和免疫疾病的研究尤为重要。流式细胞仪的用途包括基因敲除小鼠的监测及其重构、tcr转基因小鼠的分析、转移淋巴细胞亚群的跟踪、egfp标记融合蛋白的定量、T细胞mhc四聚体的分析、变异细菌的鉴定等。由于对多参数流式细胞术的不同应用的认识增加，导致仪器的可用性高度有限，因此对流式细胞术分析的需求显着增加。PI和技术总监在分析细胞学方面总共有40年的工作经验。新的3激光BD数字LSR TM将大大减少当前的等待时间，并为nih支持的不同科学家群体提供具有成本效益的高信息量的快速分析方法。激光BD数字LSR TM是最先进的，三激光，10参数，8色分析仪，具有双阈值能力，非常适合分析小颗粒。将激光BD数字LSR TM放入现有的流式细胞术设备中，将进一步提高使用效率。将该单元整合到该设施将保证由一位杰出的、经验丰富的技术总监Julie Auger进行监督和管理，并作为该机构癌症中心核心的一部分继续提供支持。将其纳入已建立的在线预订系统可确保并记录该工具的最大限度和公平使用。一个已经成立的内部咨询委员会将监督一个广泛的多学科科学家小组的公平和适当的使用，同时优先考虑NIH支持的小组。

DESCRIPTION (provided by applicant): Flow cytometry has become an indispensable tool for numerous areas of biological and medical research. Multiple parameters can be measured simultaneously on individual living cells with such a speed that an entire population of cells can be analyzed within seconds to minutes. Rare and unusual events are identified rapidly and expression levels of defined cell-surface or intracellular molecules can be quantified with great ease and precision. The ability to detect such events is particularly critical for the study of cancer and immunological diseases. Uses of the flow cytometry instrument include the monitoring of knockout mice and their reconstitution, analysis of TCR-transgenic mice, the tracking of transferred lymphoid subpopulations, quantification of EGFP-labeled fusion proteins, MHC-tetramer analyses of T cells, identification of variant bacteria, etc. The need for flow cytometric analyses has markedly increased because of an increased awareness of the diverse applications of multiparameter flow cytometry resulting in highly limited availability of the instruments. The PI and the Technical Director have a combined 40-year work experience in analytical cytology. A new 3 Laser BD Digital LSR TM will drastically reduce the current waiting times and provide a cost-efficient highly informative rapid method of analysis to a diverse group of NIH-supported scientists. The Laser BD Digital LSR TM is a state-of-the-art, triple laser, 10 parameter, 8-color analyzer, with a dual thresholding ability ideal for analysis of small particles. The efficiency of the usage of the Laser BD Digital LSR TM will be further increased by placing the unit into the existing Flow Cytometry Facility. Integration of this unit into this facility will guarantee supervision and management by an outstanding, highly experienced Technical Director, Julie Auger, and continued support as part of the institution's Cancer Center Core. Inclusion into an already established system of on-line bookings ensures and documents the maximal and fair usage of the instrument. An already established Internal Advisory Committee will oversee an equitable and appropriate use by a broad multidisciplinary group of scientists while giving priority to NIH supported groups.