Three Laser BD Digital LSR

描述(申请人提供)：流式细胞术已经成为许多生物和医学研究领域不可或缺的工具。可以在单个活细胞上同时测量多个参数，速度如此之快，可以在几秒钟到几分钟内分析整个细胞群体。罕见和不寻常的事件被快速识别，定义的细胞表面或细胞内分子的表达水平可以非常容易和精确地量化。检测这类事件的能力对于癌症和免疫性疾病的研究尤为关键。流式细胞仪的用途包括监测基因敲除小鼠及其重建、分析TCR转基因小鼠、跟踪转移的淋巴亚群、EGFP标记的融合蛋白的定量、T细胞的MHC-四聚体分析、变异细菌的鉴定等。由于人们对多参数流式细胞仪的各种应用的认识不断提高，导致仪器的可用性非常有限，因此对流式细胞仪分析的需求显著增加。PI和技术总监在分析细胞学方面有40年的工作经验。新的3激光BD数字LSR TM将大大缩短当前的等待时间，并为NIH支持的不同科学家群体提供经济高效、信息丰富的快速分析方法。激光BD Digital LSR TM是一种最先进的三激光器、10参数、8色分析仪，具有双阈值功能，非常适合分析小颗粒。通过将激光BD数字LSR TM放置到现有的流式细胞仪设备中，将进一步提高激光BD数字LSR TM的使用效率。将这一部门整合到这个设施将确保由一位杰出的、经验丰富的技术总监Julie Auger进行监督和管理，并作为该机构癌症中心核心的一部分继续提供支持。纳入已建立的在线预订系统可确保并记录对该工具的最大限度和公平使用。一个已经成立的内部咨询委员会将监督广泛的多学科科学家群体的公平和适当使用，同时优先考虑NIH支持的群体。

DESCRIPTION (provided by applicant): Flow cytometry has become an indispensable tool for numerous areas of biological and medical research. Multiple parameters can be measured simultaneously on individual living cells with such a speed that an entire population of cells can be analyzed within seconds to minutes. Rare and unusual events are identified rapidly and expression levels of defined cell-surface or intracellular molecules can be quantified with great ease and precision. The ability to detect such events is particularly critical for the study of cancer and immunological diseases. Uses of the flow cytometry instrument include the monitoring of knockout mice and their reconstitution, analysis of TCR-transgenic mice, the tracking of transferred lymphoid subpopulations, quantification of EGFP-labeled fusion proteins, MHC-tetramer analyses of T cells, identification of variant bacteria, etc. The need for flow cytometric analyses has markedly increased because of an increased awareness of the diverse applications of multiparameter flow cytometry resulting in highly limited availability of the instruments. The PI and the Technical Director have a combined 40-year work experience in analytical cytology. A new 3 Laser BD Digital LSR TM will drastically reduce the current waiting times and provide a cost-efficient highly informative rapid method of analysis to a diverse group of NIH-supported scientists. The Laser BD Digital LSR TM is a state-of-the-art, triple laser, 10 parameter, 8-color analyzer, with a dual thresholding ability ideal for analysis of small particles. The efficiency of the usage of the Laser BD Digital LSR TM will be further increased by placing the unit into the existing Flow Cytometry Facility. Integration of this unit into this facility will guarantee supervision and management by an outstanding, highly experienced Technical Director, Julie Auger, and continued support as part of the institution's Cancer Center Core. Inclusion into an already established system of on-line bookings ensures and documents the maximal and fair usage of the instrument. An already established Internal Advisory Committee will oversee an equitable and appropriate use by a broad multidisciplinary group of scientists while giving priority to NIH supported groups.