Three Laser BD Digital LSR

三激光BD数字LSR

• 批准号: 6580559
• 负责人: Hans Schreiber
• 金额: $ 21万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580559
• 关键词: biomedical equipment purchase; cell sorting; flow cytometry; lasers

DESCRIPTION (provided by applicant): Flow cytometry has become an indispensable tool for numerous areas of biological and medical research. Multiple parameters can be measured simultaneously on individual living cells with such a speed that an entire population of cells can be analyzed within seconds to minutes. Rare and unusual events are identified rapidly and expression levels of defined cell-surface or intracellular molecules can be quantified with great ease and precision. The ability to detect such events is particularly critical for the study of cancer and immunological diseases. Uses of the flow cytometry instrument include the monitoring of knockout mice and their reconstitution, analysis of TCR-transgenic mice, the tracking of transferred lymphoid subpopulations, quantification of EGFP-labeled fusion proteins, MHC-tetramer analyses of T cells, identification of variant bacteria, etc. The need for flow cytometric analyses has markedly increased because of an increased awareness of the diverse applications of multiparameter flow cytometry resulting in highly limited availability of the instruments. The PI and the Technical Director have a combined 40-year work experience in analytical cytology. A new 3 Laser BD Digital LSR TM will drastically reduce the current waiting times and provide a cost-efficient highly informative rapid method of analysis to a diverse group of NIH-supported scientists. The Laser BD Digital LSR TM is a state-of-the-art, triple laser, 10 parameter, 8-color analyzer, with a dual thresholding ability ideal for analysis of small particles. The efficiency of the usage of the Laser BD Digital LSR TM will be further increased by placing the unit into the existing Flow Cytometry Facility. Integration of this unit into this facility will guarantee supervision and management by an outstanding, highly experienced Technical Director, Julie Auger, and continued support as part of the institution's Cancer Center Core. Inclusion into an already established system of on-line bookings ensures and documents the maximal and fair usage of the instrument. An already established Internal Advisory Committee will oversee an equitable and appropriate use by a broad multidisciplinary group of scientists while giving priority to NIH supported groups.

描述（由申请人提供）：流式细胞术已成为许多生物和医学研究领域不可或缺的工具。可以同时测量单个活细胞的多个参数，其速度可以在几秒到几分钟内分析整个细胞群。可以快速识别罕见和不寻常的事件，并且可以轻松且精确地定量确定的细胞表面或细胞内分子的表达水平。检测此类事件的能力对于癌症和免疫疾病的研究尤其重要。流式细胞术仪器的用途包括监测敲除小鼠及其重建、分析TCR转基因小鼠、跟踪转移的淋巴亚群、定量EGFP标记的融合蛋白、T细胞的MHC四聚体分析、鉴定变异细菌等。由于人们对多样性的认识不断增强，对流式细胞术分析的需求显着增加。 多参数流式细胞术的应用导致仪器的可用性受到高度限制。 PI 和技术总监在分析细胞学领域合计拥有 40 年的工作经验。新型 3 Laser BD Digital LSR TM 将大大减少当前的等待时间，并为 NIH 支持的不同科学家群体提供经济高效、信息丰富的快速分析方法。 Laser BD Digital LSR TM 是一款最先进的三重激光、10 个参数、8 色分析仪，具有双阈值功能，非常适合分析小颗粒。通过将Laser BD Digital LSR TM 装置放入现有的流式细胞仪设施中，该装置的使用效率将得到进一步提高。该部门整合到该设施中将保证由一位杰出、经验丰富的技术总监 Julie Auger 进行监督和管理，并作为该机构癌症中心核心的一部分提供持续支持。纳入已建立的在线预订系统可确保并记录仪器的最大程度和公平使用。已经成立的内部咨询委员会将监督广泛的多学科科学家小组的公平和适当使用，同时优先考虑 NIH 支持的小组。