Supplement to NATIONAL CENTER FOR ADVANCED ESR TECHNOLOGY

描述(由申请人提供)：本提案是根据NCRR资金公告进行的竞争性修订：PAR-12-046，父母拨款R41RR016292资金，P41中心：国家先进ESR技术生物医学中心。这项建议是为了一个新的技术项目，该项目没有包括在母基金中，但极大地促进了母基金的目标，特别是技术研究与开发项目：利用脉冲偶极ESR技术研究蛋白质和蛋白质复合体的结构(PDS-ESR)，该项目致力于改进现有的距离测量技术，并为生物分子结构研究开发新的距离测量技术。新的项目是开发核磁共振中常见的复合脉冲方法，将其应用于ESR，它需要更短的脉冲宽度，并受到更快的自旋松弛的影响。现在可以使用新的Tektronix任意波形发生器(AWG)来调制分辨率低于ns的微波脉冲。它将使双量子相干(DQC)-ESR测距方法中的脉冲能够更好地覆盖光谱，这需要非选择性脉冲。它将使双电子-电子共振(DeER)方法所需的精心设计的选择性脉冲成为可能。 这将提高信噪比并抑制不需要的信号和伪影，从而改进这些方法。AWG将被集成到脉冲ESR光谱仪中，并适当考虑Letter的详细性能。在足够宽带宽的数字采样示波器(DSO)的帮助下，监测微波桥中关键位置的复合脉冲，将允许AWG自动重新调谐，以根据需要修改复合脉冲，以保证实际到达样品的脉冲的质量。该项目将涉及一种协同互动，由推动生物医学项目(DBP)的需求驱动，如母公司赠款中的需求。将对这种新的复合脉冲技术进行仔细的测试，以展示它如何显著改进距离测量，并允许开展包括在DBP中的新的、更具挑战性的研究。

DESCRIPTION (provided by applicant): This proposal is for a competitive revision, under NCRR funding announcement: PAR-12-046, of the parent grant R41RR016292 funding, P41 Center: National Biomedical Center for Advanced ESR Technology. This proposal is for a new technology project, not included on the parent grant, but which greatly benefits the aims of the parent grant, in particular Technology Research and Development (TR&D) Project: Structure of Proteins and Protein Complexes by Pulse Dipolar ESR (PDS-ESR), which is concerned with improving existing and developing new distance measurement techniques for structural studies on biomolecules. The new project is to develop methods of composite pulses, common in NMR, to be applied to ESR, which requires much shorter pulse widths and is subject to much faster spin-relaxation. This is now possible using a new Tektronix Arbitrary Waveform Generator (AWG) that will enable the modulation of the microwave pulses with sub-ns resolution. It will enable better spectral coverage by the pulses in the double-quantum coherence (DQC)-ESR method for distance measurements, which requires non-selective pulses. It will enable well-designed selective pulses required for the double electron-electron resonance (DEER) method. This will enhance signal-to-noise and suppression of unwanted signals and artifacts, thereby improving these methodologies. The AWG will be integrated into the pulse ESR spectrometer with due consideration of the letter's detailed performance. Monitoring of the composite pulsing at key positions in the microwave bridge, with the help of a sufficiently wide-bandwidth digital sampling oscilloscope (DSO), will permit automatic retuning of the AWG to modify as needed the composite pulses to guarantee the quality of the pulses that actually reach the sample. This project will involve a synergistic interaction, driven by the needs of the Driving Biomedical Projects (DBP's) as in the Parent Grant. Careful tests will be made of this new composite-pulse technology to demonstrate how it significantly improves the distance measurements and allows new and more challenging studies included in the DBP's to be carried out.