Supplement to NATIONAL CENTER FOR ADVANCED ESR TECHNOLOGY

国家先进 ESR 技术中心补充资料

• 批准号: 8417782
• 负责人: Jack H Freed
• 金额: $ 41.46万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8417782
• 关键词: Acquired Immunodeficiency Syndrome; Alzheimer' s Disease; Automobile Driving; Biological; Computer software; Depressive disorder; Detection; Disease; Electrons; Funding; Generations; Health; Heart Diseases; Hodgkin Disease; Hypersensitivity; Infection; Letters; Lung; Malignant Neoplasms; Measurement; Methodology; Methods; Monitor; Morphologic artifacts; National Center for Research Resources; Noise; Parkinson Disease; Performance; Phase; Physiologic pulse; Population; Positioning Attribute; Production; Proteins; Pseudomonas aeruginosa; Relaxation; Resolution; Sampling; Schizophrenia; Shapes; Signal Transduction; Structure; Techniques; Technology; Testing; United States National Institutes of Health; Virus Diseases; Width; base; combat; design; digital; improved; microwave electromagnetic radiation; nanosecond; nervous system disorder; new technology; novel; parent grant; protein complex; protein structure; quantum; research and development

DESCRIPTION (provided by applicant): This proposal is for a competitive revision, under NCRR funding announcement: PAR-12-046, of the parent grant R41RR016292 funding, P41 Center: National Biomedical Center for Advanced ESR Technology. This proposal is for a new technology project, not included on the parent grant, but which greatly benefits the aims of the parent grant, in particular Technology Research and Development (TR&D) Project: Structure of Proteins and Protein Complexes by Pulse Dipolar ESR (PDS-ESR), which is concerned with improving existing and developing new distance measurement techniques for structural studies on biomolecules. The new project is to develop methods of composite pulses, common in NMR, to be applied to ESR, which requires much shorter pulse widths and is subject to much faster spin-relaxation. This is now possible using a new Tektronix Arbitrary Waveform Generator (AWG) that will enable the modulation of the microwave pulses with sub-ns resolution. It will enable better spectral coverage by the pulses in the double-quantum coherence (DQC)-ESR method for distance measurements, which requires non-selective pulses. It will enable well-designed selective pulses required for the double electron-electron resonance (DEER) method. This will enhance signal-to-noise and suppression of unwanted signals and artifacts, thereby improving these methodologies. The AWG will be integrated into the pulse ESR spectrometer with due consideration of the letter's detailed performance. Monitoring of the composite pulsing at key positions in the microwave bridge, with the help of a sufficiently wide-bandwidth digital sampling oscilloscope (DSO), will permit automatic retuning of the AWG to modify as needed the composite pulses to guarantee the quality of the pulses that actually reach the sample. This project will involve a synergistic interaction, driven by the needs of the Driving Biomedical Projects (DBP's) as in the Parent Grant. Careful tests will be made of this new composite-pulse technology to demonstrate how it significantly improves the distance measurements and allows new and more challenging studies included in the DBP's to be carried out.

描述（由申请人提供）：本提案是根据NCRR资助公告：PAR-12-046，对母基金R41 RR 016292资助，P41中心：国家先进ESR技术生物医学中心进行竞争性修订。该提案是一项新技术项目，不包括在母赠款中，但大大有利于母赠款的目标，特别是技术研究和开发（TR&D）项目：通过脉冲偶极ESR（PDS-ESR）研究蛋白质和蛋白质复合物的结构，该项目涉及改进现有的和开发新的生物分子结构研究的距离测量技术。新的项目是开发复合脉冲的方法，在NMR中常见，将应用于ESR，这需要更短的脉冲宽度，并受到更快的自旋弛豫。现在，使用新的Tektronix任意波形发生器（AWG）可以实现这一点，该发生器将能够以低于ns的分辨率调制微波脉冲。它将使双量子相干（DQC）-ESR方法中的脉冲能够更好地覆盖光谱，用于距离测量，这需要非选择性脉冲。它将使精心设计的选择性脉冲所需的双电子-电子共振（DEER）方法。 这将增强信噪比以及对不需要的信号和伪影的抑制，从而改进这些方法。AWG将被集成到脉冲ESR光谱仪中，并适当考虑字母的详细性能。在足够宽的带宽数字采样示波器（DSO）的帮助下，在微波桥的关键位置监测复合脉冲，将允许AWG自动重新调谐，以根据需要修改复合脉冲，从而保证实际到达样品的脉冲的质量。这个项目将涉及一个协同的互动，由驱动生物医学项目（DBP）的需求驱动，如在母基金。将对这种新的复合脉冲技术进行仔细的测试，以证明它如何显着改善距离测量，并允许进行DBP中包含的新的和更具挑战性的研究。