X/Q Band Pulsed ENDOR Spectrometer

X/Q 波段脉冲 ENDOR 光谱仪

• 批准号: 9074986
• 负责人: Jack H Freed
• 金额: $ 139.81万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9074986
• 关键词: Active Sites; Anabolism; Antineoplastic Agents; Buffaloes; Chemistry; Copper; Drug Delivery Systems; Electron Nuclear Double Resonance; Electron Transport; Electrons; Enzymes; Housing; Hydration status; Hydrogenase; Iron; Lipids; Measurement; Mediating; Membrane Fusion; Minor; Nuclear; Oxidation-Reduction; Oxidoreductase; Photochemistry; Physiologic pulse; Proteins; Research; Research Project Grants; Ribonucleases; Role; Sampling; Services; Signal Transduction; Structure; Sulfur; Surface; Technical Expertise; Technology; Time; United States National Institutes of Health; Universities; biological systems; cancer therapy; cofactor; electron nuclear double resonance spectroscopy; improved; nitrification; novel

 DESCRIPTION (provided by applicant): The aim of this proposal is to establish a modern pulsed Electron-Nuclear Double Resonance (ENDOR) capability at Cornell University to be located in the National Biomedical Center for Advanced ESR Technology (ACERT). It will be used by research groups at Cornell, University of Rochester and SUNY Buffalo. ACERT possesses all the necessary expertise and technical capability to maintain the facility. The NIH-supported research projects of the major users include: (1) ENDOR study of novel chemistry of a [4Fe-4S]-cluster- containing radical enzyme in diphthamide biosynthesis; (2) ENDOR and Electron-Spin Echo Envelope Modulation (ESEEM) to probe the role of hydration in membrane fusion; (3) ENDOR spectroscopy studies of proteins that mediate signal transduction through redox and photochemistry; (4) mechanism of inhibition of ribonuclease reductase by anti-cancer drugs; and (5) structure and dynamics of porphysomes - new photo- sensitive agents in cancer therapy and drug delivery. Additional projects from minor users include: (6) structure and function of artificial hydrogenases; (7) nitrification of enzymes and electron transfer proteins; and (8) hydration at lipid surfaces. ACERT, since its establishment in 2001 provides EPR expertise and service to many research groups nationally which represent many biomedical fields. While the EPR currently performed in ACERT has been crucial in obtaining structural and functional information on paramagnetic cofactors in biological systems, such as iron- sulfur clusters, copper active sites etc., additional studies using ENDOR were often necessary but not available at Cornell for these users. Having an ENDOR shared capability at Cornell to be housed in ACERT will dramatically improve the quality of research for the groups participating in this proposal, as well as others who interact with ACERT. They will have control of their ENDOR measurements, which would become part of their daily research. Also, importantly, ENDOR and EPR studies will be carried out at the same time on the same samples and under the same conditions. As described in projects of major/minor users, ENDOR and EPR (e.g. ESEEM) are complimentary to each other in terms of revealing different aspects of interactions between the unpaired electron and nuclear spins present in the biological system studied.

 描述（由适用提供）：该提案的目的是在康奈尔大学建立现代的脉冲电子核双共振（ENDOR）能力，位于国家高级ESR技术中心（ACERT）。康奈尔大学，罗切斯特大学和纽约州立大学布法罗的研究小组将使用它。 Acert拥有维护设施的所有必要的专业知识和技术能力。 NIH支持的主要用户的研究项目包括：（1）[4FE-4S] - 簇的新化学研究 - 含Diphthamide生物合成中的自由基酶； （2）Endor和Electron-Spin Echo包膜调制（ESEEM），以探测水合在膜融合中的作用； （3）培养基通过氧化还原和光化学信号转导的蛋白质的内传光谱研究； （4）抗癌药减少核糖核酸酶抑制的机制； （5）卟啉症的结构和动力学 - 癌症治疗和药物递送的新照片敏感剂。次要用户的其他项目包括：（6）人工氢化酶的结构和功能； （7）酶和电子转移蛋白的硝化； （8）脂质表面的水合。自2001年成立以来，Acert为全国许多研究小组提供了EPR专业知识和服务，这些研究小组代表了许多生物医学领域。尽管当前在ACERT中执行的EPR对于在生物系统中获取有关顺磁性辅助因子的结构和功能信息至关重要。在康奈尔（Cornell）拥有Endor共享能力，将在ACERT中安置，这将显着提高参与该提案的小组的研究质量，以及其他与ACERT互动的人。他们将控制自己的Endor测量结果，这将成为他们日常研究的一部分。同样，重要的是，Endor和EPR研究将同时在相同的样本和相同条件下同时进行。如主要/次要用户的项目中所述，Endor和EPR（例如ESEEM）在揭示生物系统研究中存在的未配对电子和核自旋之间相互作用的不同方面相互互补。