X/Q Band Pulsed ENDOR Spectrometer

X/Q 波段脉冲 ENDOR 光谱仪

• 批准号: 9074986
• 负责人: Jack H Freed
• 金额: $ 139.81万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9074986
• 关键词: Active Sites; Anabolism; Antineoplastic Agents; Buffaloes; Chemistry; Copper; Drug Delivery Systems; Electron Nuclear Double Resonance; Electron Transport; Electrons; Enzymes; Housing; Hydration status; Hydrogenase; Iron; Lipids; Measurement; Mediating; Membrane Fusion; Minor; Nuclear; Oxidation-Reduction; Oxidoreductase; Photochemistry; Physiologic pulse; Proteins; Research; Research Project Grants; Ribonucleases; Role; Sampling; Services; Signal Transduction; Structure; Sulfur; Surface; Technical Expertise; Technology; Time; United States National Institutes of Health; Universities; biological systems; cancer therapy; cofactor; electron nuclear double resonance spectroscopy; improved; nitrification; novel

 DESCRIPTION (provided by applicant): The aim of this proposal is to establish a modern pulsed Electron-Nuclear Double Resonance (ENDOR) capability at Cornell University to be located in the National Biomedical Center for Advanced ESR Technology (ACERT). It will be used by research groups at Cornell, University of Rochester and SUNY Buffalo. ACERT possesses all the necessary expertise and technical capability to maintain the facility. The NIH-supported research projects of the major users include: (1) ENDOR study of novel chemistry of a [4Fe-4S]-cluster- containing radical enzyme in diphthamide biosynthesis; (2) ENDOR and Electron-Spin Echo Envelope Modulation (ESEEM) to probe the role of hydration in membrane fusion; (3) ENDOR spectroscopy studies of proteins that mediate signal transduction through redox and photochemistry; (4) mechanism of inhibition of ribonuclease reductase by anti-cancer drugs; and (5) structure and dynamics of porphysomes - new photo- sensitive agents in cancer therapy and drug delivery. Additional projects from minor users include: (6) structure and function of artificial hydrogenases; (7) nitrification of enzymes and electron transfer proteins; and (8) hydration at lipid surfaces. ACERT, since its establishment in 2001 provides EPR expertise and service to many research groups nationally which represent many biomedical fields. While the EPR currently performed in ACERT has been crucial in obtaining structural and functional information on paramagnetic cofactors in biological systems, such as iron- sulfur clusters, copper active sites etc., additional studies using ENDOR were often necessary but not available at Cornell for these users. Having an ENDOR shared capability at Cornell to be housed in ACERT will dramatically improve the quality of research for the groups participating in this proposal, as well as others who interact with ACERT. They will have control of their ENDOR measurements, which would become part of their daily research. Also, importantly, ENDOR and EPR studies will be carried out at the same time on the same samples and under the same conditions. As described in projects of major/minor users, ENDOR and EPR (e.g. ESEEM) are complimentary to each other in terms of revealing different aspects of interactions between the unpaired electron and nuclear spins present in the biological system studied.

 描述（由申请人提供）：本提案的目的是在康奈尔大学建立现代脉冲电子-核双共振（ENDOR）能力，该能力将位于国家生物医学中心先进ESR技术（ACERT）。它将被康奈尔大学、罗切斯特大学和纽约州立大学布法罗的研究小组使用。ACERT拥有所有必要的专业知识和技术能力来维护该设施。主要用户的NIH支持的研究项目包括：（1）含[4Fe-4S]-簇的自由基酶在联苯二甲酰胺生物合成中的新化学的ENDOR研究;（2）ENDOR和电子自旋回波包络调制（ESEEM）以探测水合作用在膜融合中的作用;（3）通过氧化还原和光化学介导信号转导的蛋白质的ENDOR光谱研究;（4）ENDOR光谱研究。（4）抗癌药物抑制核糖核酸酶还原酶的机制;（5）新型光敏抗癌药物卟啉囊泡的结构和动力学。小用户的其他项目包括：（6）人工氢化酶的结构和功能;（7）酶和电子转移蛋白质的硝化作用;（8）脂质表面的水合作用。ACERT自2001年成立以来，为代表许多生物医学领域的许多研究小组提供EPR专业知识和服务。虽然目前在ACERT中进行的EPR在获得生物系统中顺磁性辅因子（例如铁-硫簇、铜活性位点等）的结构和功能信息方面至关重要，通常需要使用ENDOR进行额外的研究，但康奈尔大学无法为这些用户提供这些研究。将康奈尔大学的ENDOR共享能力安置在ACERT中，将大大提高参与该提案的团体以及与ACERT互动的其他团体的研究质量。他们将控制ENDOR测量，这将成为他们日常研究的一部分。此外，重要的是，ENDOR和EPR研究将在相同的样品和相同的条件下同时进行。正如在主要/次要用户的项目中所描述的那样，ENDOR和EPR（例如ESEEM）在揭示所研究的生物系统中存在的未成对电子和核自旋之间相互作用的不同方面方面是互补的。