National Biomedical Center for Advanced ESR Technology (ACERT)

项目总结/摘要： 这是一个P41更新提案，以继续国家生物医学中心的先进ESR技术 （ACERT）。技术研究和开发（TR&D）的四个主要领域将得到强调。他们 包括1）高灵敏度脉冲偶极ESR谱和2D-FT ESR从X波段到Ka波段; 2）电子 用于非常小的生物样品的超高灵敏度的自旋共振; 3）高灵敏度自旋共振的发展 频率ESR，和4）改进的ESR分析和频谱处理的计算方法。 广泛驱动的生物医学项目和其他与生物医学研究人员的合作将大大受益 从这些TR& D，这反过来又是由DBP对新的和改进的ESR技术的需求驱动的。 因此，TR& D和DBP之间存在重要的协同作用。正在开发的技术 在许多方面，ACERT在世界上是独一无二的。广泛的DBP和合作/服务项目， ACERT将继续把这些现代ESR方法带到生物医学研究的最前沿，特别是 在TR&D中解决了额外的挑战。这包括我们提高光谱灵敏度的计划 在我们的ACERT光谱仪中，需求持续增长。传播计划和 培训解决了将这些新技术带到美国和其他地方的其他实验室的需要。 除了向外部科学家提供设施外，还出版和宣传研究结果， 以新方法为中心并举办讲习班。

PROJECT SUMMARY/ABSTRACT: This is a P41 renewal proposal to continue the National Biomedical Center for Advance ESR Technology (ACERT). Four principal areas of Technology Research and Development (TR&D) will be emphasized. They consist of 1) High-Sensitivity Pulse Dipolar ESR Spectroscopy and 2D-FT ESR from X to Ka Band; 2) Electron Spin Resonance with Ultra-High Sensitivity for Very Small Biological Samples; 3) Developments in High Frequency ESR, and 4) Improved Computational Methods for ESR Analysis and Spectral Processing. Extensive Driven Biomedical Projects and other collaborations with biomedical researchers will greatly benefit from these TR&Ds, which in turn are driven by the DBP needs for the new and improved ESR technologies. Thus, there is an important synergism amongst the TR&Ds and the DBPs. The technologies being developed at ACERT are in many respects unique in the world. The extensive DBP's and collaborative/service projects at ACERT will continue to bring these modern ESR methods to the forefront of biomedical research, especially as the additional challenges are addressed in the TR&D's. This includes our plans to increase spectral sensitivity in our ACERT spectrometers, for which the demands continue to grow. The plans for dissemination and training address the need to bring these new technologies to other laboratories in the USA and elsewhere in addition to making the facilities available to outside scientists, publishing and advertising the results of the Center and running workshops on the new methodologies.