Importance of pulmonary venous electrophysiology in the development of atrial fibrillation

肺静脉电生理学在心房颤动发生过程中的重要性

• 批准号: nhmrc : 244214
• 负责人: Prof Prashanthan Sanders
• 金额: $ 13.08万
• 依托单位国家: 澳大利亚
• 项目类别: Early Career Fellowships
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/importance-pulmonary-venous-atrial-fibrillation/113435
• 关键词: Importance; pulmonary; venous; electrophysiology; development

(ii) Transplant rejection can be inhibited by expression in the graft of CTLA4-Fc a reagent that blocks T cell co-stimulation enhancing allo-graft acceptance (Transplantation 2000 69:1806). High-level expression for over 100 days is expected to correlate with optimal graft acceptance. Our ability to use Kunjin to express beta galactosidase for several months in vivo without inflammation illustrates the potential for this approach (CIB ref 15). Initially we intend to use P815 cells injected i.p. into C57BL-6, where they are usually rejected within a few days. In contrast, P815 cells with Kunjin replicon-mediated CTLA4-Fc expression should survive for an extended period. Graft survival is easily monitored using FACS and anti-H-2d antibodies.

(ii)通过在移植物中表达CTLA 4-Fc可以抑制移植排斥，CTLA 4-Fc是一种阻断T细胞共刺激增强同种异体移植物接受性的试剂（Transplantation 2000 69：1806）。预计超过100天的高水平表达与最佳移植物接受相关。我们使用Kunjin在体内表达β半乳糖苷酶数月而无炎症的能力说明了这种方法的潜力（CIB参考文献15）。最初，我们打算将P815细胞腹腔注射到C57 BL-6中，在那里它们通常在几天内被排斥。相反，具有Kunjin复制子介导的CTLA 4-Fc表达的P815细胞应存活较长时间。使用FACS和抗H-2d抗体容易地监测移植物存活。