Optopathology by Light Microscopy and Molecular Techniques

光学显微镜和分子技术的光病理学

• 批准号: 8306148
• 负责人: MICHAEL J. MCKENNA
• 金额: $ 54.68万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8306148
• 关键词: Acoustic Neuroma; Acoustics; Adipose tissue; Affect; Animal Model; Atrophic; Audiometry; Auditory; Auditory Brainstem Responses; Autopsy; Behavior; Belief; Bilateral; Biopsy; Birth; Brain; Cells; Cessation of life; Clinical; Clinical Management; Cochlea; Cochlear Nerve; Collaborations; Communities; DNA; Data; Diagnosis; Disease; Doctor of Philosophy; Ear Diseases; Endosteum; Equilibrium; Excision; Fiber; Functional disorder; Future; Genomics; Goals; Guidelines; Hearing; Heart; Histologic; Human; Hyalin; Individual; Invaded; Knowledge; Laboratories; Labyrinth; Lead; Lesion; Life; Ligaments; Liquid Chromatography; Medicine; Mentorship; Methods; Mitotic; Molecular; Molecular Medicine; Molecular Target; National Institute on Deafness and Other Communication Disorders; Nature; Nerve; Neurilemmoma; Neurofibromatosis 2; Nuclear; Operative Surgical Procedures; Organ; Otolaryngology; Otology; Otosclerosis; Pancreas; Pathogenesis; Pathologic; Pathology; Pharmacotherapy; Physiology; Prevention; Process; Proteomics; Pure-Tone Audiometry; Regenerative Medicine; Registries; Research; Research Personnel; Resources; Scala Tympani; Scanning; Science; Sense Organs; Sensorineural Hearing Loss; Sensory; Severities; Supporting Cell; System; Techniques; Temporal bone structure; Testing; Time; Training; Training Programs; X-Ray Computed Tomography; base; bone; career; cell determination; clinical Diagnosis; clinical decision-making; clinical practice; design; hearing impairment; improved; insight; light microscopy; member; neoplastic cell; planetary Atmosphere; round window; tool; tumor

DESCRIPTION (provided by applicant): Knowledge of the pathologic basis of disease is central to the study of medicine, including disorders affecting the auditory and vestibular systems. Otology is unique in that the inner ear is inaccessible during life and so, conventional techniques of pathologic study such as biopsy and surgical excision are not feasible. Consequently, insight into the pathologic basis of ear disease can only be obtained by postmortem study of temporal bones and by developing credible animal models. An improved understanding of the pathology and pathogenesis of disorders affecting the auditory and vestibular systems should lead to more rational diagnosis and clinical management of these diseases. The procurement, processing and study of human temporal bones is a time-consuming and costly endeavor which lies outside the purview of clinical departments of pathology, and is therefore a research endeavor that is performed in temporal bone laboratories. This proposal was developed in keeping with the guidelines announced in RFA-DC-11-003 that is meant to establish the NIDCD Otopathology Research Collaboration Network. Our goals include the following: 1. Investigate pathology of hearing loss caused by cochleovestibular schwannoma. 2. Investigate radiologic-histologic correlations in otosclerosis, and the pathogenetic mechanism of sensorineural hearing loss when otosclerosis involves the cochlea. 3. Investigate dynamics of sensory and non-sensory cell turnover in the human inner ear. 4. Establish a program for the training of future temporal bone researchers. 5. Cooperate with the NIDCD Otopathology Research Collaboration Network. The proposed research is designed to provide new knowledge and insight into the pathology and pathophysiology of otosclerosis, acoustic tumors and cell turnover in the inner ear, which, in turn, should lead to better methods of diagnosis and therapy.

描述（由申请人提供）：了解疾病的病理基础是医学研究的核心，包括影响听觉和前庭系统的疾病。耳科的独特之处在于内耳在生活中是不可接近的，因此，传统的病理研究技术，如活检和手术切除是不可行的。因此，深入了解耳部疾病的病理基础只能通过对颞骨的死后研究和建立可靠的动物模型来获得。提高对影响听觉和前庭系统的疾病的病理和发病机制的理解将导致这些疾病的更合理的诊断和临床管理。人类颞骨的获取、加工和研究是一项耗时且昂贵的工作，超出了临床病理部门的范围，因此是一项在颞骨实验室进行的研究工作。该提案是根据RFA-DC-11-003中宣布的指南制定的，旨在建立NIDCD耳病理学研究合作网络。我们的目标包括：探讨耳蜗前庭神经鞘瘤致听力损失的病理特点。2. 探讨耳硬化的影像学-组织学相关性，以及当耳硬化累及耳蜗时感音神经性听力损失的发病机制。3. 研究人内耳感觉和非感觉细胞转换的动力学。4. 建立培养未来颞骨研究人员的计划。5. 与NIDCD耳病理研究合作网络合作。本研究旨在为耳硬化、听力学肿瘤和内耳细胞转换的病理和病理生理学提供新的认识和见解，从而为更好的诊断和治疗方法提供依据。