Optopathology by Light Microscopy and Molecular Techniques

光学显微镜和分子技术的光病理学

• 批准号: 8519980
• 负责人: MICHAEL J. MCKENNA
• 金额: $ 52.2万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8519980
• 关键词: Acoustic Neuroma; Acoustics; Adipose tissue; Affect; Animal Model; Atrophic; Audiometry; Auditory; Auditory Brainstem Responses; Autopsy; Behavior; Belief; Bilateral; Biopsy; Birth; Brain; Cells; Cessation of life; Clinical; Clinical Management; Cochlea; Cochlear Nerve; Collaborations; Communities; DNA; Data; Diagnosis; Disease; Doctor of Philosophy; Ear Diseases; Endosteum; Equilibrium; Excision; Fiber; Functional disorder; Future; Genomics; Goals; Guidelines; Hearing; Heart; Histologic; Human; Hyalin; Individual; Invaded; Knowledge; Laboratories; Labyrinth; Lead; Lesion; Life; Ligaments; Liquid Chromatography; Medicine; Mentorship; Methods; Mitotic; Molecular; Molecular Medicine; Molecular Target; National Institute on Deafness and Other Communication Disorders; Nature; Nerve; Neurilemmoma; Neurofibromatosis 2; Nuclear; Operative Surgical Procedures; Organ; Otolaryngology; Otology; Otosclerosis; Pancreas; Pathogenesis; Pathologic; Pathology; Pharmacotherapy; Physiology; Prevention; Process; Proteomics; Pure-Tone Audiometry; Regenerative Medicine; Registries; Research; Research Personnel; Resources; Scala Tympani; Scanning; Science; Sense Organs; Sensorineural Hearing Loss; Sensory; Severities; Supporting Cell; System; Techniques; Temporal bone structure; Testing; Time; Training; Training Programs; X-Ray Computed Tomography; base; bone; career; cell determination; clinical Diagnosis; clinical decision-making; clinical practice; design; hearing impairment; improved; insight; light microscopy; member; neoplastic cell; planetary Atmosphere; round window; tool; tumor

DESCRIPTION (provided by applicant): Knowledge of the pathologic basis of disease is central to the study of medicine, including disorders affecting the auditory and vestibular systems. Otology is unique in that the inner ear is inaccessible during life and so, conventional techniques of pathologic study such as biopsy and surgical excision are not feasible. Consequently, insight into the pathologic basis of ear disease can only be obtained by postmortem study of temporal bones and by developing credible animal models. An improved understanding of the pathology and pathogenesis of disorders affecting the auditory and vestibular systems should lead to more rational diagnosis and clinical management of these diseases. The procurement, processing and study of human temporal bones is a time-consuming and costly endeavor which lies outside the purview of clinical departments of pathology, and is therefore a research endeavor that is performed in temporal bone laboratories. This proposal was developed in keeping with the guidelines announced in RFA-DC-11-003 that is meant to establish the NIDCD Otopathology Research Collaboration Network. Our goals include the following: 1. Investigate pathology of hearing loss caused by cochleovestibular schwannoma. 2. Investigate radiologic-histologic correlations in otosclerosis, and the pathogenetic mechanism of sensorineural hearing loss when otosclerosis involves the cochlea. 3. Investigate dynamics of sensory and non-sensory cell turnover in the human inner ear. 4. Establish a program for the training of future temporal bone researchers. 5. Cooperate with the NIDCD Otopathology Research Collaboration Network. The proposed research is designed to provide new knowledge and insight into the pathology and pathophysiology of otosclerosis, acoustic tumors and cell turnover in the inner ear, which, in turn, should lead to better methods of diagnosis and therapy.

描述（由申请人提供）：了解疾病的病理基础是医学研究的核心，包括影响听觉和前庭系统的疾病。耳科的独特之处在于内耳在生命中是不可接近的，因此，常规的病理研究技术如活检和手术切除是不可行的。因此，只有通过对颞骨的死后研究和建立可靠的动物模型，才能深入了解耳部疾病的病理基础。对影响听觉和前庭系统的疾病的病理学和发病机制的更好理解应该导致这些疾病的更合理的诊断和临床管理。人类颞骨的获取、处理和研究是一项耗时且昂贵的奋进，其超出了病理学临床部门的范围，因此是在颞骨实验室中进行的研究奋进。该提案是根据RFA-DC-11-003中宣布的旨在建立NIDCD耳病理学研究合作网络的指导方针制定的。我们的目标包括：1.耳蜗前庭神经鞘瘤致听力损失的病理学研究。2.研究耳硬化症的放射学-组织学相关性，以及耳硬化症累及耳蜗时感音神经性听力损失的发病机制。3.研究人类内耳感觉和非感觉细胞更新的动力学。4.建立一个培训未来颞骨研究人员的计划。5.与NIDCD耳病理学研究合作网络合作。拟议的研究旨在为耳硬化症，听肿瘤和内耳细胞周转的病理学和病理生理学提供新的知识和见解，这反过来又会导致更好的诊断和治疗方法。