Molecular Basis of Otopathology

耳病理学的分子基础

• 批准号: 6773301
• 负责人: MICHAEL J. MCKENNA
• 金额: $ 33.67万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6773301
• 关键词: biomarker; bone metabolism; clinical research; collagen; cytokine; ear pharmacology; extracellular matrix proteins; gene expression; genetically modified animals; histology; human subject; immunocytochemistry; measles virus; molecular pathology; osteoblasts; osteocytes; otosclerosis; parathyroid hormones; patient oriented research; physiologic bone resorption; polymerase chain reaction; tissue /cell culture; virus cytopathogenic effect

DESCRIPTION (provided by applicant): The goal of the proposed investigation is to achieve a better understanding of the molecular pathology of otosclerosis and ultimately develop better forms of treatment and prevention. The specific aims are to investigate bone metabolism of the otic capsule of normal mice and mice with genetic defects which give rise to otosclerosis in humans. We will apply what we learn from the mouse model to achieve a better understanding of factors which may result in abnormal otic capsule remodeling in humans. To accomplish this we will obtain bone specimens from normal patients and patients with otosclerosis, and study osteoblast bone cells in culture. We will apply what we learn from the mouse studies to normal human otic capsule physiology and determine which molecular factors are important in the initiation of otic capsule remodeling. Specifically, we will study defects in type 1 collagen gene expression and determine what effects these defects have on osteoblast physiology that may lead to the development of otosclerosis. If successful, the proposed study will result in a better understanding of the molecular factors involved in the development of otosclerosis, the development of genetic tests that will allow for identification of individuals who are at risk for developing otosclerosis, and an in vitro method for evaluating potential therapeutic agents.

描述（由申请人提供）：拟议研究的目标是更好地了解耳硬化症的分子病理学，并最终开发更好的治疗和预防形式。具体目的是研究正常小鼠和具有引起人类耳硬化症的遗传缺陷的小鼠的耳囊的骨代谢。我们将应用我们从小鼠模型中学到的知识，以更好地了解可能导致人类异常耳囊重塑的因素。为了实现这一目标，我们将从正常患者和耳硬化症患者中获得骨标本，并在培养中研究成骨细胞。我们将把从小鼠研究中学到的知识应用于正常的人类耳囊生理学，并确定哪些分子因素在耳囊重塑的启动中是重要的。具体来说，我们将研究1型胶原基因表达的缺陷，并确定这些缺陷对成骨细胞生理学的影响，可能导致耳硬化症的发展。如果成功，拟议的研究将导致更好地了解参与耳硬化症发展的分子因素，发展基因检测，以识别有耳硬化症风险的个体，以及评估潜在治疗药物的体外方法。